Study type: Medical/biological study (experimental study)

Non-thermal effects of continuous 2.45 GHz microwaves on Fas-induced apoptosis in human Jurkat T-cell line. med./bio.

Published in: Bioelectrochemistry 2000; 51 (2): 157-161

Aim of study (acc. to author)

To investigate the effects of microwave exposure on apoptosis in human Jurkat cells.

Background/further details

Apoptosis is among other things induced by Fas. Fas/Apo 1 (CD95) belongs to the tumor necrosis factors (TNF). One way Fas acts is by elevating the ceramide synthesis. Also butyrate is known to suppress T cell viability through apoptotic mechanism.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.45 GHz
Modulation type: CW
Exposure duration: continuous for 48 h

Exposure 1

Main characteristics
Frequency 2.45 GHz
Type
Waveform
Exposure duration continuous for 48 h
Modulation
Modulation type CW
Exposure setup
Exposure source
Chamber cells placed in a 60 l Plexiglas bacteriological incubator with 5% CO2 - 95% air mixture
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
power density 5 mW/cm² - - - -
SAR 4 W/kg - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Butyrate- and ceramide-induced apoptosis was not altered during microwave exposure. Microwave exposure did not alter the cell proliferation but slightly enhance the sensitivity to Fas-induced apoptosis. Microwave exposure leads only to a temperature increase of 0.1°C. However, temperature conditions of 37.5°C did not show any changes. The results suggest that the observed effects of microwave exposure on Fas-induced apoptosis in Jurkat cells are related to non-thermal mechanism.

Study character:

Study funded by

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