Study type: Medical/biological study (experimental study)

Extremely low frequency magnetic field induced changes in motor behaviour of gerbils submitted to global cerebral ischemia. med./bio.

Published in: Behav Brain Res 2012; 228 (2): 241-246

Aim of study (acc. to author)

To investigate whether the exposure to an extremely low frequency magnetic field affects the behavior of Mongolian gerbils submitted to global cerebral ischemia.

Background/further details

Cerebral ischemia was induced by surgical occlusion of both common carotid arteries for 10 min. Adult (3-month-old) male gerbils were divided into four groups (6-8 animals/group): 1.) control, 2.) exposure, 3.) ischemia, and 4.) ischemia + exposure. The control group was further subdivided into intact controls, sham operated, and sham exposed animals.
The behavioral parameters were investigated on days 1, 2, 4, and 7 of exposure (immediate effect) and seven days after exposure (day 14, delayed effect).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 7 days

General information

animals were divided into the following four groups: i) control ii) exposure to EMF iii) ischemia iv) ischenia + exposure to EMF

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 7 days
Exposure setup
Exposure source
Setup two 26 cm x 43 cm x 15 cm polycarbonate cages with 3 - 4 animals each, positioned on both sides of the electromagnet so that the center of the cage was at a distance of 20 cm from the pole
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.2 mT minimum measured - at the end of the cage with the greatest distance to the pole
magnetic flux density 2 mT maximum measured - at the end of the cage with the smallest distance to the pole
magnetic flux density 0.5 mT spatial average measured - at the center of the cage

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

The 10-min global cerebral ischemia per se induced a significant increase of motor activity, stereotypy and rotations, and consequently immobility decrease until day 4 in ischemic animals compared to control gerbils. Exposure to an extremely low frequency magnetic field per se showed a tendency of increasing motor activity (data not shown).
Exposure to an extremely low frequency magnetic field + ischemia (group 4) inhibited the development of ischemia-induced motor hyperactivity during the whole period of monitoring, particularly in the first two days after reperfusion, when the post-ischemic hyperactivity was most evident.
The authors concluded that the exposure to an extremely low frequency magnetic field decreased motor hyperactivity induced by the 10-min global cerebral ischemia.

Study character:

Study funded by

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