Study type: Medical/biological study (experimental study)

Effect of 300 mT static and 50 Hz 0.1 mT extremely low frequency magnetic fields on Tuber borchii mycelium. med./bio.

Published in: Can J Microbiol 2012; 58 (10): 1174-1182

Aim of study (acc. to author)

To examine the effect of exposure to a static magnetic field or an extremely low frequency magnetic field on gene expression, carbohydrate metabolism and hyphal growth in truffle mycelium.

Background/further details

Truffle is a very expensive edible mushroom, which cultivation is difficult. Therefore, the exposure to magnetic fields could represent a possibility to improve cultivation strategies.
Hyphae are long, branching filamentous structures of the fungi.

Endpoint

Exposure

Exposure Parameters
Exposure 1:
Exposure duration: continuous for 1 h/day for 3 days
Exposure 2: 50 Hz
Exposure duration: continuous for 1 h/day for 3 days

Exposure 1

Main characteristics
Frequency
Type
Exposure duration continuous for 1 h/day for 3 days
Exposure setup
Exposure source
Setup 10 mm thick neodymium disks with a diameter of 28.85 mm
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 300 mT peak value - - in the middle of the culture

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 1 h/day for 3 days
Exposure setup
Exposure source
Chamber mycelium (21 day-cultured) placed in 50 ml flasks inside the containers (unit 1)
Setup exposure apparatus consisted of 4 units, allowing uniform exposure to magnetic B-field (unit 1), electric E-field (unit 2), mutually orthogonal E- and B-fields (unit 3), and no field (unit 4); Faraday shields excluded electric noise in the units; each unit had 4 rectangular containers filled with circulating distilled water for thermalization (24°C); only unit 1 and unit 4 were used; unit 1 consisted of three 40 cm x 40 cm parallel copper plates; B-field parallel to the plates' surfaces
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.1 mT - measured - -

Reference articles

  • Potenza L et al. (2010): Effects of a 300 mT static magnetic field on human umbilical vein endothelial cells.
  • Potenza L et al. (2004): Effects of a static magnetic field on cell growth and gene expression in Escherichia coli.
  • Dacha M et al. (1993): Studies on the possible biological effects of 50 Hz electric and/or magnetic fields: evaluation of some glycolytic enzymes, glycolytic flux, energy and oxido-reductive potentials in human erythrocytes exposed in vitro to power frequency fields.
  • Miller DL et al. (1989): Addition of magnetic field capability to existing extremely-low-frequency electric field exposure systems.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In cell cultures exposed to extremely low frequency magnetic fields, the gene expression of all examined genes was significantly upregulated compared to the control group. However, no significant differences regarding gene expression were observed between the static magnetic field exposure and the control group.
The enzyme activities of hexokinase, glucose phosphate isomerase, pyruvate kinase and glucose-6-phosphate dehydrogenase were significantly increased in the cell cultures exposed to extremely low frequency magnetic fields in comparison to the control group, while in the static magnetic field exposed group only the enzyme activities of hexokinase and glucose-6-phosphate dehydrogenase were significantly increased.
No differences were observed regarding hyphal growth.
The authors conclude that exposure to extremely low frequency magnetic fields induced changes in gene expression and metabolism of the truffle mycelium and could be a possibility to improve prospective cultivation methods.

Study character:

Study funded by

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