研究のタイプ: 医学／生物学の研究 (experimental study)

[白トリュフ（セイヨウショウロ子実体）に対する300mTの静磁界および50Hz、0.1mTの超低周波磁界の影響] med./bio.

Effect of 300 mT static and 50 Hz 0.1 mT extremely low frequency magnetic fields on Tuber borchii mycelium

著者: Potenza L, Saltarelli R, Polidori E, Ceccaroli P, Amicucci A, Zeppa S, Zambonelli A, Stocchi V
掲載誌: Can J Microbiol 2012; 58 (10): 1174-1182
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この研究は、静磁界（SMF）および超低周波磁界（ELF-MF）へのばく露が白トリュフ（Tuber borchii）の菌糸成長における生体分子レベルの変化を誘発できるか否かを調べた。菌糸体に、SMF（300 mT）またはELF-MF（0.1 mT、50Hz）のばく露を1日1時間で連続3日間与えた。その後、遺伝子発現および生化学的分析を行った。その結果、ELF-MFばく露を受けた菌糸体では、定量的リアルタイムポリメラーゼ連鎖反応で、菌糸成長に関与するいくつかの遺伝子の発現上昇が検出され、多くの解糖系酵素の活性が上昇した；対照的に、SMFばく露を受けた菌糸体では、遺伝子発現に違いは観察されず、グルコース6-リン酸デヒドロゲナーゼおよびヘキソキナーゼの活性のみが上昇した、と報告している。

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研究目的（著者による）

To examine the effect of exposure to a static magnetic field or an extremely low frequency magnetic field on gene expression, carbohydrate metabolism and hyphal growth in truffle mycelium.

詳細情報

Truffle is a very expensive edible mushroom, which cultivation is difficult. Therefore, the exposure to magnetic fields could represent a possibility to improve cultivation strategies.
Hyphae are long, branching filamentous structures of the fungi.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1:
  • DC/static
ばく露時間: continuous for 1 h/day for 3 days
ばく露2: 50 Hz
ばく露時間: continuous for 1 h/day for 3 days

ばく露1

主たる特性
周波数
  • DC/static
タイプ
  • magnetic field
ばく露時間 continuous for 1 h/day for 3 days
ばく露装置
ばく露の発生源／構造
  • neodymium disks
ばく露装置の詳細 10 mm thick neodymium disks with a diameter of 28.85 mm
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 300 mT peak - - in the middle of the culture

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 1 h/day for 3 days
ばく露装置
ばく露の発生源／構造
  • parallel copper plates
チャンバの詳細 mycelium (21 day-cultured) placed in 50 ml flasks inside the containers (unit 1)
ばく露装置の詳細 exposure apparatus consisted of 4 units, allowing uniform exposure to magnetic B-field (unit 1), electric E-field (unit 2), mutually orthogonal E- and B-fields (unit 3), and no field (unit 4); Faraday shields excluded electric noise in the units; each unit had 4 rectangular containers filled with circulating distilled water for thermalization (24°C); only unit 1 and unit 4 were used; unit 1 consisted of three 40 cm x 40 cm parallel copper plates; B-field parallel to the plates' surfaces
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 0.1 mT - 測定値 - -

Reference articles

  • Potenza L et al. (2010): [ヒト臍静脈内皮細胞に対する300 mT静磁界の影響]
  • Potenza L et al. (2004): [大腸菌の細胞増殖と遺伝子発現に対する直流磁界の影響]
  • Dacha M et al. (1993): [50 Hz電界と磁界の生物影響に関する研究：商用周波電磁界にばく露されたヒト赤血球での糖分解酵素、エネルギー、酸化-還元電位の評価]
  • Miller DL et al. (1989): [既存の極低周波電界ばく露システムへの磁界ばく露システムの追加]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

In cell cultures exposed to extremely low frequency magnetic fields, the gene expression of all examined genes was significantly upregulated compared to the control group. However, no significant differences regarding gene expression were observed between the static magnetic field exposure and the control group.
The enzyme activities of hexokinase, glucose phosphate isomerase, pyruvate kinase and glucose-6-phosphate dehydrogenase were significantly increased in the cell cultures exposed to extremely low frequency magnetic fields in comparison to the control group, while in the static magnetic field exposed group only the enzyme activities of hexokinase and glucose-6-phosphate dehydrogenase were significantly increased.
No differences were observed regarding hyphal growth.
The authors conclude that exposure to extremely low frequency magnetic fields induced changes in gene expression and metabolism of the truffle mycelium and could be a possibility to improve prospective cultivation methods.

研究の種別:

研究助成

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