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To examine the possibility to enhance the cell differentiation of human mesenchymal stem cells through pulsed extremely low frequency magnetic fields.
周波数 | 75 Hz |
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タイプ |
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波形 |
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ばく露時間 | 8 hours/day for up to 21 days |
Additional information | square wave |
ばく露の発生源/構造 | |
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チャンバの詳細 | cells were cultured in six-well culture plates and placed inside an incubator at equal distance from two solenoids |
ばく露装置の詳細 | solenoids were arranged in parallel with one another and perpendicular to the support base |
Sham exposure | A sham exposure was conducted. |
Morphological changes indicating differentiation into cartilage cells were more evident and earlier in cells treated with pulsed magnetic fields compared with the untreated ones. The expression of typ II collagen and the secretion of proteoglycans were significantly increased in the exposed cell cultures compared to the untreated cell cultures.
The authors conclude that exposure of human mesenchymal stem cells to pulsed extremely low frequency magnetic fields could enhance cell differentiation into cartilage cells.
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