Study type: Medical/biological study (experimental study)

ELF magnetic fields do not affect cell survival and DNA damage induced by ultraviolet B. med./bio.

Published in: Bioelectromagnetics 2014; 35 (2): 108-115

Aim of study (acc. to author)

To determine whether exposure of skin derived cells to an extremely low frequency magnetic field alters the cell viability and the DNA damage induced by ultraviolet B.

Background/further details

Xeroderma pigmentosum is a rare hereditary disease of the skin characterized by a low ability to repair DNA damage induced by ultraviolet radiation. The XP2OS(SV)-cell line was established from xeroderma pigmentosum skin. The W138VA13-cell line was used as a control group.
First, the cells were radiated with ultraviolet B of different intensities (0, 20, 40, 60 or 80 J/m²). Afterwards, the cells were exposed to an extremely low frequency field or sham exposed for 1, 3, or 24 hours.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous for 1, 3 or 24 hours

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration continuous for 1, 3 or 24 hours
Exposure setup
Exposure source
Setup exposure system consisted of CO2 incubator with a built-in magnetic field, constructed with Helmholtz coils (inner diameter 250 mm, length 160 mm, 128 turns), a slide regulator and a thermo controller; temperature in incubtaor: 37 ± 0.2°C; interior and exterieur were shielded by silicon steel and Permalloy C, respectively; direction of magnetic field was vertical
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5 mT - measured - -
electric field strength 0.047 V/m - calculated - cell culture dish (100 mm)
electric field strength 0.007 V/m - calculated - 24-well plate

Reference articles

  • Sakurai T et al. (2004): An extremely low frequency magnetic field attenuates insulin secretion from the insulinoma cell line, RIN-m.
  • Miyakoshi J et al. (1996): Exposure to magnetic field (5 mT at 60 Hz) does not affect cell growth and c-myc gene expression.
  • Misakian M et al. (1993): Biological, physical, and electrical parameters for in vitro studies with ELF magnetic and electric fields: a primer.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In both cell lines, ultraviolet radiation reduced the cell viability and induced DNA damage. However, no significant influence of magnetic field exposure on the cell viability and DNA damage was observed.
The data indicate that the exposure to extremely low frequency magnetic fields had no influence on the cell viability and the DNA damage induced by ultraviolet B.

Study character:

Study funded by

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