Study type: Medical/biological study (experimental study)

Effects of in vitro exposure to power frequency magnetic fields on UV-induced DNA damage of rat lymphocytes. med./bio.

Published in: Bioelectromagnetics 2004; 25 (7): 560-562

Aim of study (acc. to author)

To investigate the effects of 50/60 Hz magnetic fields (similar to those emitted by power frequency) on DNA damage, induced by UV rays, in rat lymphocytes.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 60 min
Exposure 2: 50 Hz
Exposure duration: 5 min

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration 60 min
Exposure setup
Exposure source
Setup 2 ml of suspensions of lymphocytes were placed on open petri dishes (3.5 cm in diameter) with the half of them placed in the bath inside the coils.
Additional info The other half of Perti dishes were placed in the bath outside the coils. Both samples outside and inside the coil system were irradiated to UV (ultaviolet radiation) for 5 min at a flux density of 0.5 W/m². Two controls were used: one exposed to MF (magnetic field) without UV and the second was neither exposed to UV or MF.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 40 µT effective value measured - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Exposure duration 5 min
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 40 µT effective value measured - -

Reference articles

  • Zmyslony M et al. (2000): DNA damage in rat lymphocytes treated in vitro with iron cations and exposed to 7 mT magnetic fields (static or 50 Hz).

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Rat lymphocytes were irradiated in vitro by UV and the effects of a 50 Hz magnetic field on the oxidative damage of DNA were examined. It was found that an UV-irradiation followed by a magnetic field exposure increases DNA damage. The results suggest that magnetic field exposure affects the process of DNA repair in a negative way.

Study character:

Study funded by

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