Study type: Medical/biological study (experimental study)

Exposure to magnetic field (5 mT at 60 Hz) does not affect cell growth and c-myc gene expression. med./bio.

Published in: J Radiat Res 1996; 37 (3): 185-191

Aim of study (acc. to author)

To examine the effects of extremely low frequency magnetic fields on cell growth and c-myc gene expression in CHO cells (Chinese hamster ovary) in vitro.

Background/further details

To study the cell growth rate CHO cells were exposed for up to 130 hours.
To determine c-myc mRNA expression CHO cells were exposed for 3 hours. The positive controls were treated with TPA. Each experiment was repeated three times.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: up to 130 h

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration up to 130 h
Exposure setup
Exposure source
Chamber CO2 incubator with a built-in magnet generator using two Helmholtz coils
Setup coils of 128 turns, 250 mm inner diameter and 160 mm apart; cylindrical exposure space (14 cm height x 20 cm diameter) housing sixteen 10 or four 15 cm culture dishes
Additional info inside and outside of the incubator shielded by silicon steel and Permalloy C
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5 mT effective value measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

No significant differences in the growth rate and the c-myc expression of CHO cells were observed with 5 mT extremely low frequency magnetic field exposure, sham exposure and incubation in a conventional incubator.

Study character:

Study funded by

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