Study type: Medical/biological study (experimental study)

Oxidative changes and apoptosis induced by 1800-MHz electromagnetic radiation in NIH/3T3 cells. med./bio.

Published in: Electromagn Biol Med 2015; 34 (1): 85-92

Aim of study (acc. to author)

The effects of exposure of mouse embryonic fibroblasts to a 1800 MHz electromagnetic field on oxidative stress, DNA damage and apoptosis should be investigated.

Background/further details

Cells were divided into the following groups: exposure to the electromagnetic field for 1) 0.5 h, 2) 1 h, 3) 1.5 h, 4) 2 h, 5) 4 h, 6) 6 h or 7) 8 hours. For each group, a corresponding sham exposure group was created.
Positive controls were conducted and each experiment was repeated thrice.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 1,800 MHz
Modulation type: pulsed
Exposure duration: intermittent exposure (5 min on/10 min off) for 0.5, 1, 1.5, 2, 4, 6 or 8 hours

Exposure 1

Main characteristics
Frequency 1,800 MHz
Type
Exposure duration intermittent exposure (5 min on/10 min off) for 0.5, 1, 1.5, 2, 4, 6 or 8 hours
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional info

GSM signal

Exposure setup
Exposure source
Chamber culture dishes
Setup two waveguides, one for exposure and the other one for sham exposure, were placed in incubator; culture dishes in dish holder were placed inside the waveguide in the H-field maximum of the standing wave and E-polarization; the temperature was kept at 37 ± 0.1°C
Sham exposure A sham exposure was conducted.
Additional info temperature differences between the exposed and the sham exposed cultures never exceeded 0.1°C
Parameters
Measurand Value Type Method Mass Remarks
SAR 2 W/kg mean calculated - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

There were no significant differences in the amount of DNA damages between the exposure groups and their corresponding sham exposure groups.
The intracellular level of reactive oxygen species was significantly increased after 1, 4 and 8 hours of exposure compared to the corresponding sham exposure groups.
After exposure for 1, 4 or 8 h, the number of late-apoptotic cells increased significantly compared to the respective sham-exposed groups as well.
The authors conclude that exposure of mouse embryonic fibroblasts to a 1800 MHz electromagnetic field could promote oxidative stress and apoptosis.

Study character:

Study funded by

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