Study type: Medical/biological study (experimental study)

Effects of combined radiofrequency radiation exposure on levels of reactive oxygen species in neuronal cells. med./bio.

Published in: J Radiat Res 2014; 55 (2): 265-276

Aim of study (acc. to author)

To examine the effects of an exposure to combined radiofrequency electromagnetic fields (CDMA and WCDMA) on the level of reactive oxygen species in different neuronal cells.

Background/further details

Two experiments were performed. In the first experiment, the cells were treated as follows and the level of reactive oxygen species was analyzed: 1 a) incubator control group, 1 b) sham exposure, 1 c) exposure to CDMA and WCDMA. In the second experiment, cells were co-exposed to reactive oxygen species inducers (H2O2 and menadione) and the level of reactive oxygen species as well as the cell viability were determined: 2 a) incubator control group, 2 b) incubator control + H2O2 (0-1000 µM) or menadione (0-400 µM), 2 c) sham exposure + H2O2 (0-1000 µM) or menadione (0-400 µM) and 2 d) exposure to CDMA und WCDMA + H2O2 (0-1000 µM) or menadione (0-400 µM).
For the experiments, 3 different cancer cell lines (neuronal cells) were used (see "exposed system"). Additionally, NIH3T3 cells (mouse fibroblasts) were used as control cells. After the radiofrequency exposure, cells were analyzed at 1, 3, 6 and 12 h after H2O2 treatment or after 0.5, 1 and 3 hours after menadione treatment.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 837–1,950 MHz
Exposure duration: continuous for 2 hours
combined exposure to CDMA and WCDMA

Exposure 1

Main characteristics
Frequency 837–1,950 MHz
Type
Exposure duration continuous for 2 hours
Additional info combined exposure to CDMA and WCDMA
Exposure setup
Exposure source
Chamber 8 petri dishes per exposure, temperature of exposure chamber: 37 ±0.3 °C (monitored twice per second), mixture of air and CO2 (5 % CO2 inside the chamber)
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 2 W/kg - - - CDMA signal
SAR 2 W/kg - - - WCDMA signal

Reference articles

  • Lee KY et al. (2011): Effects of combined radiofrequency radiation exposure on the cell cycle and its regulatory proteins.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In the first experiment, no consistent significant differences occurred in the levels of reactive oxygen species over the time in the radiofrequency exposed neuronal cell lines compared to the corresponding incubator control group and the sham exposed cell cultures.
In the second experiment, the radiofrequency exposed neuronal cells with H2O2 or menadione showed no significant changes compared to cell cultures treated with H2O2 or menadione alone.
The data indicate that neither exposure to radiofrequency fields (CDMA and WCDMA) alone nor in combination with H2O2 or menadione alters the level of reactive oxygen species in neuronal cells.

Study character:

Study funded by

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