この研究は、1800-MHz GSM通話モードの電磁界（EMR）ばく露（平均SAR 2W/kg、5分ON/10分OFFの断続、ばく露時間は0.5から8時間まで）を与えたマウス胚由来線維芽細胞（NIH/3T3）での活性酸素種（ROS）、DNA損傷、アポトーシスを調べた。その結果、EMRばく露後に細胞内ROSレベルは有意に上昇し、ばく露時間1時間で最大レベルに達し、それ以上のばく露時間（8時間まで）では徐々にレベルは低下した；γH2AXの数に対する有意な影響は見られなかった；EMRばく露群では擬似ばく露群に比べ、アポトーシスの進行した細胞の割合が高かった、と報告している。
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The effects of exposure of mouse embryonic fibroblasts to a 1800 MHz electromagnetic field on oxidative stress, DNA damage and apoptosis should be investigated.
Cells were divided into the following groups: exposure to the electromagnetic field for 1) 0.5 h, 2) 1 h, 3) 1.5 h, 4) 2 h, 5) 4 h, 6) 6 h or 7) 8 hours. For each group, a corresponding sham exposure group was created.
Positive controls were conducted and each experiment was repeated thrice.
|ばく露時間||intermittent exposure (5 min on/10 min off) for 0.5, 1, 1.5, 2, 4, 6 or 8 hours|
|Repetition frequency||217 Hz|
|ばく露装置の詳細||two waveguides, one for exposure and the other one for sham exposure, were placed in incubator; culture dishes in dish holder were placed inside the waveguide in the H-field maximum of the standing wave and E-polarization; the temperature was kept at 37 ± 0.1°C|
|Sham exposure||A sham exposure was conducted.|
|Additional information||temperature differences between the exposed and the sham exposed cultures never exceeded 0.1°C|
There were no significant differences in the amount of DNA damages between the exposure groups and their corresponding sham exposure groups.
The intracellular level of reactive oxygen species was significantly increased after 1, 4 and 8 hours of exposure compared to the corresponding sham exposure groups.
After exposure for 1, 4 or 8 h, the number of late-apoptotic cells increased significantly compared to the respective sham-exposed groups as well.
The authors conclude that exposure of mouse embryonic fibroblasts to a 1800 MHz electromagnetic field could promote oxidative stress and apoptosis.