研究のタイプ: 医学／生物学の研究 (experimental study)

[1800 MHz電磁界によりNIH/3T3細胞に誘発される酸化的変化とアポトーシス] med./bio.

Oxidative changes and apoptosis induced by 1800-MHz electromagnetic radiation in NIH/3T3 cells

著者: Hou Q, Wang M, Wu S, Ma X, An G, Liu H, Xie F
掲載誌: Electromagn Biol Med 2015; 34 (1): 85-92
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この研究は、1800-MHz GSM通話モードの電磁界（EMR）ばく露（平均SAR 2W/kg、5分ON/10分OFFの断続、ばく露時間は0.5から8時間まで）を与えたマウス胚由来線維芽細胞（NIH/3T3）での活性酸素種（ROS）、DNA損傷、アポトーシスを調べた。その結果、EMRばく露後に細胞内ROSレベルは有意に上昇し、ばく露時間1時間で最大レベルに達し、それ以上のばく露時間（8時間まで）では徐々にレベルは低下した；γH2AXの数に対する有意な影響は見られなかった；EMRばく露群では擬似ばく露群に比べ、アポトーシスの進行した細胞の割合が高かった、と報告している。

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研究目的（著者による）

The effects of exposure of mouse embryonic fibroblasts to a 1800 MHz electromagnetic field on oxidative stress, DNA damage and apoptosis should be investigated.

詳細情報

Cells were divided into the following groups: exposure to the electromagnetic field for 1) 0.5 h, 2) 1 h, 3) 1.5 h, 4) 2 h, 5) 4 h, 6) 6 h or 7) 8 hours. For each group, a corresponding sham exposure group was created.
Positive controls were conducted and each experiment was repeated thrice.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,800 MHz
Modulation type: pulsed
ばく露時間: intermittent exposure (5 min on/10 min off) for 0.5, 1, 1.5, 2, 4, 6 or 8 hours
  • SAR: 2 W/kg mean

ばく露1

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
ばく露時間 intermittent exposure (5 min on/10 min off) for 0.5, 1, 1.5, 2, 4, 6 or 8 hours
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional information

GSM signal
ばく露装置
ばく露の発生源／構造
チャンバの詳細 culture dishes
ばく露装置の詳細 two waveguides, one for exposure and the other one for sham exposure, were placed in incubator; culture dishes in dish holder were placed inside the waveguide in the H-field maximum of the standing wave and E-polarization; the temperature was kept at 37 ± 0.1°C
Sham exposure A sham exposure was conducted.
Additional information temperature differences between the exposed and the sham exposed cultures never exceeded 0.1°C
パラメータ
測定量 値 種別 Method Mass 備考
SAR 2 W/kg mean 計算値 - -

Reference articles

  • Schuderer J et al. (2004): [1800 MHzのインビトロ実験に用いる強力なばく露および環境制御機能を備えた高尖頭SARばく露装置]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

There were no significant differences in the amount of DNA damages between the exposure groups and their corresponding sham exposure groups.
The intracellular level of reactive oxygen species was significantly increased after 1, 4 and 8 hours of exposure compared to the corresponding sham exposure groups.
After exposure for 1, 4 or 8 h, the number of late-apoptotic cells increased significantly compared to the respective sham-exposed groups as well.
The authors conclude that exposure of mouse embryonic fibroblasts to a 1800 MHz electromagnetic field could promote oxidative stress and apoptosis.

研究の種別:

研究助成

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