Study type: Medical/biological study (experimental study)

Action of a high-frequency magnetic field on the cartilage matrix in vitro. med./bio.

Published in: Bioelectrochem Bioenerg 1995; 37 (1): 25-29

Aim of study (acc. to author)

To investigate the chondrogenic effects (synthesis and turnover of cartilage matrix components) of electromagnetic fields, using organoid cultures of blastemal cells from limb buds of mouse embryos.

Background/further details

For simulating inflammatory conditions in vitro the cells were treated with bacterial lipopolysaccharides from day 4 onwards.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 150 MHz
Modulation type: AM
Exposure duration: continuous for 4 h
Exposure 2: 150 MHz
Modulation type: AM
Exposure duration: continuous for 24 h
Exposure 3: 150 MHz
Modulation type: AM
Exposure duration: repeated daily exposure, 4 h/day for 7 days

Exposure 1

Main characteristics
Frequency 150 MHz
Type
Exposure duration continuous for 4 h
Modulation
Modulation type AM
Modulation frequency 16 Hz
Exposure setup
Exposure source
Chamber Electromagnetic chamber maintained at 37°C, 95% humidity and 5% CO2
Setup Culture dishes were placed in an electromagnetic exposure chamber.
Additional info All cultures including the controls, which were placed in the same incubator without exposure to magnetic field, were treated with 10 mg/ml LPS (bacterial lipopolysaccharides) from day 4 of the culture.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 4 mV/cm mean unspecified - -
magnetic flux density 40 nT unspecified unspecified - -

Exposure 2

Main characteristics
Frequency 150 MHz
Type
Exposure duration continuous for 24 h
Modulation
Modulation type AM
Modulation frequency 16 Hz
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 4 mV/cm mean estimated - -
magnetic flux density 40 nT unspecified unspecified - -

Exposure 3

Main characteristics
Frequency 150 MHz
Type
Exposure duration repeated daily exposure, 4 h/day for 7 days
Modulation
Modulation type AM
Modulation frequency 16 Hz
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 4 mV/cm mean unspecified - -
magnetic flux density 40 nT unspecified unspecified - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

High-frequency magnetic fields seem to cause a moderate enhancement of collagens and proteoglycans in spite of the indirect catabolic effects of lipopolysaccharides by enhancing the activity of metalloproteinases. The slight inhibitory effect of magnetic fields on metalloproteinases is too weak to reduce matrix degradation markedly, thus leading to accumulation of collagens. It is concluded that high-frequency magnetic fields exert anabolic effects on embryonal chondrogenic tissue in vitro.

Study character:

Study funded by

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