Study type: Medical/biological study (experimental study)

Organ culture studies on the development of mouse embryo limb buds under EMF influence. med./bio.

Published in: Int J Radiat Biol 2006; 82 (7): 455-464

Aim of study (acc. to author)

To study the morphological and histological modifications induced by an electromagnetic field on the development of the limb bud in vitro.

Background/further details

20 limb buds per group (exposed, sham-exposed, control) were used.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 2 h

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 2 h
Exposure setup
Exposure source
Setup The culture dishes were placed inside a plastic basket which was placed inside the coil mounted vertically in the incubator. The coils were turned off during the sham exposure.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 13.1 mT unspecified measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The growth rate in both fore and hindlimb buds (in proximal-distal and anterior-posterior axes) was significantly increased in exposed limb buds. Chondrocyte counts and mitotic figures of mesenchymal and red blood cells were significantly increased as compared with those of sham-exposed and control groups. There was also a significant reduction of mesenchymal cell counts, while no significant difference was found in the degenerated cell counts among the three groups.
These results suggest that electromagnetic fields, under the conditions applied, has progressive effects on the limb bud development and that both proliferation and cell differentiation can be stimulated in vitro. The authors suggest a chondrogenic potential for electromagnetic fields which may be useful for increasing the cell proliferation and regeneration of chondrogenic cells.

Study character:

Study funded by

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