In a previous study, the authors found that extremely low frequency magnetic fields caused a cell cycle arrest potentially through the activation of the ATM-Chk2-p21 pathway in human keratinocyte HaCaT cells (Huang et al.).
Ionizing radiation (UV-B, 21.5 J/m²) was performed as a positive control. Cell proliferation and cell viability experiments were additionally performed with HaCaT cells.
Exposure duration: continuous up to 144 hours (24, 48, 72, 96, 120, 144 hours)
|Exposure duration||continuous up to 144 hours (24, 48, 72, 96, 120, 144 hours)|
|Setup||coil system generated a uniform 1.5 mT magnetic field; temperature inside the incubator was 36.9 ± 0.3°C|
|Sham exposure||A sham exposure was conducted.|
|Additional info||all sham exposed cells were cultured in a chamber magnetically shielded using a mumetal box in the same incubator in which the exposed cells were incubated|
|magnetic flux density||1.5 mT||-||-||-||-|
Extremely low frequency magnetic fields do not influence cell proliferation, cell viability, cell cycle distribution as well as gene expression and protein expression in keratinocytes derived from neonatal foreskin when compared to sham exposure. However, cell proliferation and cell viability were significantly decreased in exposed HaCaT cells in comparison to sham exposed ones. Moreover, an additional experiment with simultaneous exposure of the two cell lines showed that the keratinocytes derived from neonatal foreskin and the HaCaT cells exhibited distinct responses to the magnetic field.
The authors conclude that the biological effects of extremely low frequency magnetic fields are cell type specific. These findings could present an explanation for the inconsistent results in previous studies using various experimental models.