研究のタイプ: 医学／生物学の研究 (experimental study)

[異なる種類の表皮ケラチノサイトは超低周波電磁界に異なる反応を示す] med./bio.

Distinct Epidermal Keratinocytes Respond to Extremely Low-Frequency Electromagnetic Fields Differently

著者: Huang CY, Chuang CY, Shu WY, Chang CW, Chen CR, Fan TC, Hsu IC
掲載誌: PLoS One 2014; 9 (11): e113424-1-e113424-7
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この研究は、著者らの先行研究で磁界（1.5 mT、60 Hz）の影響が観察されたヒトケラチノサイト細胞株HaCaTに代えて、別のヒトケラチノサイト細胞株である正常ヒト表皮ケラチノサイト細胞株NHEKを用いて同じ実験デザインで実験した。その結果、細胞の成長、増殖、細胞周期分布、ATM信号伝達経路の活性化への影響が何も観察されなかった。この2種類の細胞株で磁界の影響が異なることを確認するため、同じ培養器内においてHaCaTとNHEKに同時に磁界ばく露した結果、細胞成長への影響が異なった､と報告している。

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研究目的（著者による）

To investigate whether extremely low frequency magnetic fields influence the cell cycle in normal epidermal keratinocytes.

詳細情報

In a previous study, the authors found that extremely low frequency magnetic fields caused a cell cycle arrest potentially through the activation of the ATM-Chk2-p21 pathway in human keratinocyte HaCaT cells (Huang et al.).
Ionizing radiation (UV-B, 21.5 J/m²) was performed as a positive control. Cell proliferation and cell viability experiments were additionally performed with HaCaT cells.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: continuous up to 144 hours (24, 48, 72, 96, 120, 144 hours)

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous up to 144 hours (24, 48, 72, 96, 120, 144 hours)
ばく露装置
ばく露の発生源／構造
ばく露装置の詳細 coil system generated a uniform 1.5 mT magnetic field; temperature inside the incubator was 36.9 ± 0.3°C
Sham exposure A sham exposure was conducted.
Additional information all sham exposed cells were cultured in a chamber magnetically shielded using a mumetal box in the same incubator in which the exposed cells were incubated
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 1.5 mT - - - -
Additional parameter details

in unexposed environemt: 1.5 µT± 0.03 µT

Reference articles

  • Huang CY et al. (2014): [超低周波電磁界はATM-Chk2-p21経路の活性化を通してG1期停止を引き起こす]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Extremely low frequency magnetic fields do not influence cell proliferation, cell viability, cell cycle distribution as well as gene expression and protein expression in keratinocytes derived from neonatal foreskin when compared to sham exposure. However, cell proliferation and cell viability were significantly decreased in exposed HaCaT cells in comparison to sham exposed ones. Moreover, an additional experiment with simultaneous exposure of the two cell lines showed that the keratinocytes derived from neonatal foreskin and the HaCaT cells exhibited distinct responses to the magnetic field.
The authors conclude that the biological effects of extremely low frequency magnetic fields are cell type specific. These findings could present an explanation for the inconsistent results in previous studies using various experimental models.

研究の種別:

研究助成

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