Study type: Medical/biological study (experimental study)

Effects of long-term 50 Hz power-line frequency electromagnetic field on cell behavior in Balb/c 3T3 cells med./bio.

Published in: PLoS One 2015; 10 (2): e0117672

Aim of study (acc. to author)

To investigate the effects of long-term 50 Hz magnetic field exposure on cell behavior (cell proliferation, cell viability, cell cycle, cell transformation, cell morphology) of mouse fibroblasts.

Background/further details

Mouse fibroblasts were exposed or sham exposed during the exponential growth phase. Rat glioma cells (C6 cells) were used as a positive control in the plate assay and human esophageal squamous cell carcinoma cells (KYSE150 cells) were used as a positive control in the soft agar assay.



Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 2 h/day, 5 days/week for 11 weeks

Exposure 1

Main characteristics
Frequency 50 Hz
Exposure duration 2 h/day, 5 days/week for 11 weeks
Exposure setup
Exposure source
Setup setup consisted of 2 four-coil systems each placed inside a mu-metal box, respectively; both systems were inside a commercial incubator; temperature was 37°C; atmosphere consisted of 95% air, 5% CO2 and 100% relative humidity; temperature was monitored at the location of the flasks during exposure with Pt100 probes; two fans per box were mounted to guarantee enough atmospheric exchange of the exposure chambers
Sham exposure A sham exposure was conducted.
Additional info current in the bifilar coils could be switched parallel for field exposure or non-parallel for sham exposure
Measurand Value Type Method Mass Remarks
magnetic flux density 2.3 mT effective value measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No significant changes were observed between exposed and sham exposed mouse fibroblasts regarding cell morphology, apoptosis, cell migration ability (scratch test) and cell transformation. However, the cell viability and the protein expression of PCNA and CyclinD1 were significantly decreased in the exposed group compared to the sham exposure. Additionally, the cell cycle distribution was significantly changed: After eleven weeks of exposure, the percentage of cells in the G1 phase and in the G2 phase was increased compared to the sham exposed cells while the percentage of cells in the S phase was decreased.
The authors conclude that long-term 50 Hz magnetic field exposure could affect cell viability and cell cycle by down-regulation of the proteins PCNA and CyclinD1.

Study character:

Study funded by

Related articles