Study type: Medical/biological study (experimental study)

Electromagnetic field of extremely low frequency (60Hz and 120Hz) effects the cell cycle progression and the metabolic activity of the anterior pituitary gland cells in vitro. med./bio.

Published in: IEEE EUROCON 2017 -17th International Conference on Smart Technologies, Ohrid. IEEE, 2017: 537-541, ISBN 9781509038442

Aim of study (acc. to author)

The effects of exposure of pig anterior pituitary cells to a 60 Hz or 120 Hz magnetic field on cell cycle progression and cell viability should be investigated.

Background/further details

The cells were divided into the following groups: 1) exposure to a 60 Hz magnetic field with 2 - 6 mT, 2) 1) exposure to a 120 Hz magnetic field with 2 - 6 mT, 3) exposure to a 60 Hz magnetic field with 2 - 6 mT and subsequent further incubation without exposure for 24 hours, 4) exposure to a 120 Hz magnetic field with 2 - 6 mT and subsequent further incubation without exposure for 24 hours. A separate control group was used for each exposure condition.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: 2 hours
Exposure 2: 120 Hz
Exposure duration: 2 hours

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Exposure duration 2 hours
Exposure setup
Exposure source
  • generator (Magneris)
Chamber cells were seeded in 96-well plates
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT - - - -
magnetic flux density 2.5 mT - - - -
magnetic flux density 3 mT - - - -
magnetic flux density 4 mT - - - -
magnetic flux density 6 mT - - - -

Exposure 2

Main characteristics
Frequency 120 Hz
Type
Exposure duration 2 hours
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT - - - -
magnetic flux density 2.5 mT - - - -
magnetic flux density 3 mT - - - -
magnetic flux density 4 mT - - - -
magnetic flux density 6 mT - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In all groups, the proportion of cells in the G0/G1 phase was significantly increased compared to the respective control group, while the proportion of cells in the S phase was significantly reduced. The effects of the 60 Hz magnetic fields (groups 1 and 3) were significantly stronger compared to the 120 Hz groups (groups 2 and 4). (Note EMF-Portal: No information was provided on the magnetic flux density used in cell cycle progression experiments.) These results indicated an anti-proliferative effect of magnetic field exposure.
Cell viability was significantly increased in all groups and at all magnetic flux densities compared to the control groups.
The authors conclude that exposure of pig anterior pituitary cells to a 60 Hz or 120 Hz magnetic field could have an anti-proliferative effect by influencing cell cycle progression.

Study character:

Study funded by

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