The effects of exposure of osteoblast-like and slime mold cells to a static or 50 Hz magnetic field on the oxidation status of cytochrome oxidase should be investigated.
Cytochrome oxidase causes an redox reaction cycle in mitochondria, which is an important indicator for cellular activity as it is directly bound to oxygen consumption and ATP release of the cell.
Slime mold cells were exposed to visible light, to a 50 Hz magnetic field or a static magnetic field. The osteoblast-like cells were exposed either to a 50 Hz magnetic field or to a static magnetic field.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
Exposure duration:
continuous for 10 min
slime mold cells
|
|
|
Exposure 2:
50 Hz
Exposure duration:
continuous for 10 min
slime mold cells
|
|
|
Exposure 3:
Exposure duration:
intermittent 5 times in a row with different duration
osteoblast-like cells
|
|
|
Exposure 4:
50 Hz
Exposure duration:
intermittent 5 times in a row with different duration
osteoblast-like cells
|
|
| Frequency | |
|---|---|
| Type | |
| Exposure duration | continuous for 10 min |
| Additional info | slime mold cells |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 5 T | - | - | - | - |
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | continuous for 10 min |
| Additional info | slime mold cells |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 1 mT | minimum | - | - | - |
| magnetic flux density | 6 mT | maximum | - | - | - |
| Frequency | |
|---|---|
| Type | |
| Exposure duration | intermittent 5 times in a row with different duration |
| Additional info | osteoblast-like cells |
| Exposure source | |
|---|---|
| Setup | the sample was placed in the bore of a superconductive magnet (13 x 24 cm) together with the fiber optics; exposure setup was covered with a piece of cloth for shading |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 5 T | - | - | - | - |
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform |
|
| Exposure duration | intermittent 5 times in a row with different duration |
| Additional info | osteoblast-like cells |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 10 mT | - | - | - | - |
In slime mold cells, cytochrome oxidase showed a fast periodically fluctuating natural redox reaction cycle in the cells before the exposure. During and after exposure to visible light, the cyclic cytochrome oxidase turnover was either enhanced or suppressed with the same probability of 50%, respectively. During and after the exposure to the 50 Hz or static magnetic field this turnover was again either enhanced or suppressed (however, at least for the 50 Hz magnetic field not significantly), but with a tendency towards a suppressing effect.
Before exposure, osteoblast-like cells showed a noticeable slower natural redox reaction cycle of the cytochrome oxidase compared to the slime mold cells and a reproducible suppression of the cyclic turnover during the exposure to the 50 Hz or static magnetic field could be found. (Remark by the EMF-Portal: with one exception, no statements are made on the statistical significance of these results.)
The authors conclude, that exposure of osteoblast-like and slime mold cells to a static or 50 Hz magnetic field might influence the redox reaction cycle of the cytochrome oxidase.
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