Study type: Medical/biological study (experimental study)

Magnetic field effects on mitochondrion-activity-related optical properties in slime mold and bone forming cells. med./bio.

Published in: No authors listed, 2013. 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), Osaka IEEE, 2013: 1442-1445, ISBN-13 978-1-4577-0216-7, ISBN-10 1457702169

Aim of study (acc. to author)

The effects of exposure of osteoblast-like and slime mold cells to a static or 50 Hz magnetic field on the oxidation status of cytochrome oxidase should be investigated.

Background/further details

Cytochrome oxidase causes an redox reaction cycle in mitochondria, which is an important indicator for cellular activity as it is directly bound to oxygen consumption and ATP release of the cell.
Slime mold cells were exposed to visible light, to a 50 Hz magnetic field or a static magnetic field. The osteoblast-like cells were exposed either to a 50 Hz magnetic field or to a static magnetic field.

Endpoint

Exposure

Exposure Parameters
Exposure 1:
Exposure duration: continuous for 10 min
slime mold cells
Exposure 2: 50 Hz
Exposure duration: continuous for 10 min
slime mold cells
Exposure 3:
Exposure duration: intermittent 5 times in a row with different duration
Exposure 4: 50 Hz
Exposure duration: intermittent 5 times in a row with different duration

Exposure 1

Main characteristics
Frequency
Type
Exposure duration continuous for 10 min
Additional info slime mold cells
Exposure setup
Exposure source
Chamber slime mold was exposed in 35 mm a agar plate
Setup the sample was placed in the bore of a superconductive magnet (13 x 24 cm) together with the fiber optics; exposure setup was covered with a piece of cloth for shading
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5 T - - - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 10 min
Additional info slime mold cells
Exposure setup
Exposure source
Chamber slime mold was exposed in 35 mm a agar plate
Setup 5 circular coils enclosing the sample together with the fiber optics; exposure setup was covered with a piece of cloth for shading
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT minimum - - -
magnetic flux density 6 mT maximum - - -

Exposure 3

Main characteristics
Frequency
Type
Exposure duration intermittent 5 times in a row with different duration
Additional info osteoblast-like cells
Exposure setup
Exposure source
Setup the sample was placed in the bore of a superconductive magnet (13 x 24 cm) together with the fiber optics; exposure setup was covered with a piece of cloth for shading
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5 T - - - -

Exposure 4

Main characteristics
Frequency 50 Hz
Type
Waveform
  • rectangular
Exposure duration intermittent 5 times in a row with different duration
Additional info osteoblast-like cells
Exposure setup
Exposure source
Setup 5 circular coils enclosing the sample together with the fiber optics; exposure setup was covered with a piece of cloth for shading
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 10 mT - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • before exposure
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

In slime mold cells, cytochrome oxidase showed a fast periodically fluctuating natural redox reaction cycle in the cells before the exposure. During and after exposure to visible light, the cyclic cytochrome oxidase turnover was either enhanced or suppressed with the same probability of 50%, respectively. During and after the exposure to the 50 Hz or static magnetic field this turnover was again either enhanced or suppressed (however, at least for the 50 Hz magnetic field not significantly), but with a tendency towards a suppressing effect.
Before exposure, osteoblast-like cells showed a noticeable slower natural redox reaction cycle of the cytochrome oxidase compared to the slime mold cells and a reproducible suppression of the cyclic turnover during the exposure to the 50 Hz or static magnetic field could be found. (Remark by the EMF-Portal: with one exception, no statements are made on the statistical significance of these results.)
The authors conclude, that exposure of osteoblast-like and slime mold cells to a static or 50 Hz magnetic field might influence the redox reaction cycle of the cytochrome oxidase.

Study character:

Study funded by

Related articles