研究のタイプ: 医学/生物学の研究 (experimental study)

[エンドウの根の細胞におけるCa2+含有量に対する低レベルマイクロ波および磁界の影響] med./bio.

Magnetic field effects on mitochondrion-activity-related optical properties in slime mold and bone forming cells.

掲載誌: 2013. 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), Osaka IEEE, 2013: 1442-1445, ISBN 9781457702167

この研究は、時間変化する磁界および強い静磁界の下で、Cyto-aa3酸化の細胞レベルの応答をリアルタイムで調べた。粘菌Physarum polycephalumおよび骨形成細胞MC-3T3-E1の2種類の細胞を実験に用いた。Cyto-aa3の酸化レベルは、690nm、780nm、830nmの光吸収によって計算した。ソレノイドコイルまたは5Tの超伝導磁石の口径内に光刺激用の光ファイバーを取り付けた。時間変化する磁界用のソレノイドコイルは6mTの磁界を生じた。粘菌はCyto-aa3酸化の周期的な変化を示し、Cyto-aa3の酸化還元周期は見かけ上、可視光を粘菌照射した際に変化した。光の場合と同様に、時間変化する磁界による刺激は、10分間の刺激中および刺激後に酸化還元周期を変化させた。MC-3T3-E1細胞集合でも同じ現象が観察されたが、その周期は比較的ランダムであった。最大5Tの磁界ばく露は、骨形成細胞におけるCyto-aa3振動の明確な抑制を示した。最大5Tのばく露を5回反復した場合、Cyto-aa3酸化における変化は再現性を持って生じた。

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研究目的(著者による)

The effects of exposure of osteoblast-like and slime mold cells to a static or 50 Hz magnetic field on the oxidation status of cytochrome oxidase should be investigated.

詳細情報

Cytochrome oxidase causes an redox reaction cycle in mitochondria, which is an important indicator for cellular activity as it is directly bound to oxygen consumption and ATP release of the cell.
Slime mold cells were exposed to visible light, to a 50 Hz magnetic field or a static magnetic field. The osteoblast-like cells were exposed either to a 50 Hz magnetic field or to a static magnetic field.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1:
  • DC/static
ばく露時間: continuous for 10 min
slime mold cells
ばく露2: 50 Hz
ばく露時間: continuous for 10 min
slime mold cells
ばく露3:
  • DC/static
ばく露時間: intermittent 5 times in a row with different duration
osteoblast-like cells
ばく露4: 50 Hz
ばく露時間: intermittent 5 times in a row with different duration
osteoblast-like cells

ばく露1

主たる特性
周波数
  • DC/static
タイプ
  • magnetic field
ばく露時間 continuous for 10 min
Additional information slime mold cells
ばく露装置
ばく露の発生源/構造
  • superconductive magnet
チャンバの詳細 slime mold was exposed in 35 mm a agar plate
ばく露装置の詳細 the sample was placed in the bore of a superconductive magnet (13 x 24 cm) together with the fiber optics; exposure setup was covered with a piece of cloth for shading
パラメータ
測定量 種別 Method Mass 備考
磁束密度 5 T - - - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 10 min
Additional information slime mold cells
ばく露装置
ばく露の発生源/構造
チャンバの詳細 slime mold was exposed in 35 mm a agar plate
ばく露装置の詳細 5 circular coils enclosing the sample together with the fiber optics; exposure setup was covered with a piece of cloth for shading
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT minimum - - -
磁束密度 6 mT maximum - - -

ばく露3

主たる特性
周波数
  • DC/static
タイプ
  • magnetic field
ばく露時間 intermittent 5 times in a row with different duration
Additional information osteoblast-like cells
ばく露装置
ばく露の発生源/構造
  • superconductive magnet
ばく露装置の詳細 the sample was placed in the bore of a superconductive magnet (13 x 24 cm) together with the fiber optics; exposure setup was covered with a piece of cloth for shading
パラメータ
測定量 種別 Method Mass 備考
磁束密度 5 T - - - -

ばく露4

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • rectangular
ばく露時間 intermittent 5 times in a row with different duration
Additional information osteoblast-like cells
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 5 circular coils enclosing the sample together with the fiber optics; exposure setup was covered with a piece of cloth for shading
パラメータ
測定量 種別 Method Mass 備考
磁束密度 10 mT - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露前
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

In slime mold cells, cytochrome oxidase showed a fast periodically fluctuating natural redox reaction cycle in the cells before the exposure. During and after exposure to visible light, the cyclic cytochrome oxidase turnover was either enhanced or suppressed with the same probability of 50%, respectively. During and after the exposure to the 50 Hz or static magnetic field this turnover was again either enhanced or suppressed (however, at least for the 50 Hz magnetic field not significantly), but with a tendency towards a suppressing effect.
Before exposure, osteoblast-like cells showed a noticeable slower natural redox reaction cycle of the cytochrome oxidase compared to the slime mold cells and a reproducible suppression of the cyclic turnover during the exposure to the 50 Hz or static magnetic field could be found. (Remark by the EMF-Portal: with one exception, no statements are made on the statistical significance of these results.)
The authors conclude, that exposure of osteoblast-like and slime mold cells to a static or 50 Hz magnetic field might influence the redox reaction cycle of the cytochrome oxidase.

研究の種別:

研究助成

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