Study type: Medical/biological study (experimental study)

Power-Frequency Magnetic Field Inhibits Adipogenic Differentiation in Human ADSC. med./bio.

Published in: Cell Physiol Biochem 2015; 37 (6): 2297-2310

Aim of study (acc. to author)

The effects of exposure of human mesenchymal stem cells derived from fat tissue to a 50 Hz magnetic field on the differentiation into fat cells should be investigated.

Background/further details

The study was conducted to investigate the influence of magnetic fields on semicircular lipoatrophy, a medical condition, which is characterized by semicircular dimples, mainly at the thighs.
Mesenchymal stem cells were isolated from fat tissue of two healthy donors and cells were cultivated for 5 days. Afterwards, cells received differentiation medium to induce differentiation into fat cells and were divided into the following groups: 1) cultivation for 2 weeks and subsequent exposure to the magnetic field for 42 hours, 2) direct exposure to the magnetic field for 42 hours, 3) direct exposure to the magnetic field for 42.5 hours. For each group a separate sham exposure was conducted.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: intermittent (3 h on/3 h off) for 42 hours
Exposure 2: 50 Hz
Exposure duration: intermittent (3 h on/3 h off) for 42.5 hours

Exposure 1

Main characteristics
Frequency 50 Hz
Waveform
Polarization
Exposure duration intermittent (3 h on/3 h off) for 42 hours
Exposure setup
Exposure source
Chamber cell culture dishes or microplates wit 24 wells in magnetically shielded chamber made of co-netic alloy in CO2 incubator
Setup two 1000-turn, 20-cm diameter coils made of enamelled copper wire, aligned coaxially 10 cm apart and oriented to produce vertically polarized MF inside the incubator with a 5% CO2, 37°C and 90% humidity atmosphere
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 100 µT - measured - ± 0.1 µT in region of cells

Exposure 2

Main characteristics
Frequency 50 Hz
Waveform
Polarization
Exposure duration intermittent (3 h on/3 h off) for 42.5 hours
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 100 µT - measured - ± 0.1 µT in region of cells

Reference articles

  • Trillo MA et al. (2012): Influence of a 50 Hz magnetic field and of all-transretinol on the proliferation of human cancer cell lines.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Cells exposed to the magnetic field from the beginning of differentiation (group 2) showed significantly less lipid accumulations than the respective sham exposure group. A later exposure (group 1) did not show significant differences in the amount of lipids compared to the sham exposure.
Both, immunofluorescence assay and Western blot, showed a significantly decreased protein expression of PPARγ and a significantly increased protein expression of phosphorylated ERK 1/2 and Sox9.
The authors conclude that exposure of human mesenchymal stem cells derived from fat tissue to a 50 Hz magnetic field could impair the differentiation into fat cells with participation of the PPARγ/ERK1/2 signaling pathway. This could be an indication for the etiology of semicircular lipoatrophy.

Study character:

Study funded by

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