研究のタイプ: 医学/生物学の研究 (experimental study)

[電力周波磁界はヒトADSCにおける脂肪細胞への分化を阻害する] med./bio.

Power-Frequency Magnetic Field Inhibits Adipogenic Differentiation in Human ADSC.

掲載誌: Cell Physiol Biochem 2015; 37 (6): 2297-2310

この研究は、半円状脂肪組織萎縮症(SL)と電力周波磁界MF)への職業ばく露との関連についての報告を踏まえ、脂肪組織由来間葉幹細胞(ADSC)の脂肪生成に対する50Hz、100µTのMFばく露の影響を調べた。ペトリ皿中の細胞へのばく露は42時間断続的に行った。その結果、ばく露群では細胞内脂肪蓄積が有意に減少した;細胞継代数が早い方が影響への感受性が高かった;この影響は、初期脂肪生成の主なエフェクタ/レギュレータであるPPARγの発現有意な変化を伴った、と報告している。

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研究目的(著者による)

The effects of exposure of human mesenchymal stem cells derived from fat tissue to a 50 Hz magnetic field on the differentiation into fat cells should be investigated.

詳細情報

The study was conducted to investigate the influence of magnetic fields on semicircular lipoatrophy, a medical condition, which is characterized by semicircular dimples, mainly at the thighs.
Mesenchymal stem cells were isolated from fat tissue of two healthy donors and cells were cultivated for 5 days. Afterwards, cells received differentiation medium to induce differentiation into fat cells and were divided into the following groups: 1) cultivation for 2 weeks and subsequent exposure to the magnetic field for 42 hours, 2) direct exposure to the magnetic field for 42 hours, 3) direct exposure to the magnetic field for 42.5 hours. For each group a separate sham exposure was conducted.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: intermittent (3 h on/3 h off) for 42 hours
ばく露2: 50 Hz
ばく露時間: intermittent (3 h on/3 h off) for 42.5 hours

ばく露1

主たる特性
周波数 50 Hz
波形
  • sinusoidal
偏波
  • linear
ばく露時間 intermittent (3 h on/3 h off) for 42 hours
ばく露装置
ばく露の発生源/構造
チャンバの詳細 cell culture dishes or microplates wit 24 wells in magnetically shielded chamber made of co-netic alloy in CO2 incubator
ばく露装置の詳細 two 1000-turn, 20-cm diameter coils made of enamelled copper wire, aligned coaxially 10 cm apart and oriented to produce vertically polarized MF inside the incubator with a 5% CO2, 37°C and 90% humidity atmosphere
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 100 µT - 測定値 - ± 0.1 µT in region of cells

ばく露2

主たる特性
周波数 50 Hz
波形
  • sinusoidal
偏波
  • linear
ばく露時間 intermittent (3 h on/3 h off) for 42.5 hours
ばく露装置
ばく露の発生源/構造
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 100 µT - 測定値 - ± 0.1 µT in region of cells

Reference articles

  • Trillo MA et al. (2012): [ヒトがん細胞株の増殖に対する50Hz磁界及びオールトランスレチノールの影響力]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Cells exposed to the magnetic field from the beginning of differentiation (group 2) showed significantly less lipid accumulations than the respective sham exposure group. A later exposure (group 1) did not show significant differences in the amount of lipids compared to the sham exposure.
Both, immunofluorescence assay and Western blot, showed a significantly decreased protein expression of PPARγ and a significantly increased protein expression of phosphorylated ERK 1/2 and Sox9.
The authors conclude that exposure of human mesenchymal stem cells derived from fat tissue to a 50 Hz magnetic field could impair the differentiation into fat cells with participation of the PPARγ/ERK1/2 signaling pathway. This could be an indication for the etiology of semicircular lipoatrophy.

研究の種別:

研究助成

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