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Medical/biological study (experimental study)

Rat adipose-derived mesenchymal stem cells aging reduction by zinc sulfate under extremely low frequency electromagnetic field exposure is associated with increased telomerase reverse transcriptase gene expression.

Published in: Vet Res Forum 2017; 8 (2): 89-96

Aim of study (acc. to author)

To investigate the effects of a 50 Hz magnetic field and zinc sulfate on aging and gene expression of telomerase reverse transcriptase (TERT) in rat adipose-derived mesenchymal stem cells.
Background/further details: In a pre-experiment, the optimal concentration of zinc sulfate (ZnSO4) to enhance cell growth was found to be 0.14 µg/ml. Therefore, the following groups were examined: 1) sham exposure, 2) magnetic field exposure, 3) sham exposure + 0.14 µg/ml ZnSO4, and 4) magnetic field exposure + 0.14 mg/ml ZnSO4.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 30 minutes per day for 21 consecutive days
Exposure 1
Main characteristics
Frequency 50 Hz
Type
Exposure duration 30 minutes per day for 21 consecutive days
Exposure setup
Exposure source
Setup MF was generated by a parallel set of Helmholtz coils (diameter 27 cm, respectively) with 500 turns of 0.70 mm coated copper wire; coils were placed in a cell incubator; 6-well plates were placed in the middle between the coils to receive a uniform field
Sham exposure A sham exposure was conducted.
Additional info sham exposed plates were kept under the same conditions (CO2 concentration, temperature and humidity) in another incubator without MF
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 20 mT - measured - -
Reference articles
  • Yan J et al. (2010): Effects of extremely low-frequency magnetic field on growth and differentiation of human mesenchymal stem cells.
Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated material:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The mRNA level of TERT was significantly reduced after exposure (group 2) compared to sham exposure (group 1) while the number of beta-galactosidase positive cells was significantly increased. A combination of zinc sulfate and magnetic field exposure (group 4) significantly lowered the expression level compared to zinc sulfate alone (group 3) but remained significantly increased compared to sham exposure (group 1). Similarly, but vice versa, a combination of zinc sulfate and magnetic field exposure (group 4) significantly increased the number of beta-galactosidase positive cells compared to zinc sulfate alone (group 3), but the number remained significantly lowered compared to sham exposure (group 1).
The authors conclude that exposure of rat adipose-derived mesenchymal stem cells to 50 Hz magnetic fields could affect cellular aging and that an addition of zinc sulfate could attenuate these alterations.
Study character:

Study funded by

  • University of Tabriz, Iran

Related articles

  • Fathi E et al. (2017): Enhancement of osteogenic differentiation of rat adipose tissue-derived mesenchymal stem cells by zinc sulphate under electromagnetic field via the PKA, ERK1/2 and Wnt/β-catenin signaling pathways.
  • Martinez MA et al. (2015): Power-Frequency Magnetic Field Inhibits Adipogenic Differentiation in Human ADSC.
  • Razavi S et al. (2014): Extremely low-frequency electromagnetic field influences the survival and proliferation effect of human adipose derived stem cells.