Lymphocytes and fibroblasts were used and respectively five groups were examined for every cell line and antioxidant: 1) control group without magnetic field exposure, 2) magnetic field exposure, 3) magnetic field exposure with 10 µmol/l Vit E (or 2 µmol/l Vit C), 4) magnetic field exposure with 25 µmol/l Vit E (or 10 µmol/l Vit C) and 5) magnetic field exposure with 50 µmol/l Vit E (or 100 µmol/l Vit C). The antioxidants were added six hours before exposure.
Exposure duration: continuously for 16 hours
|Exposure duration||continuously for 16 hours|
|magnetic flux density||2.3 mT||unspecified||unspecified||-||-|
In exposed fibroblasts (group 2), the percentage of apoptotic cells was higher compared to the respective control group (group 1). The addition of vitamin C (2 and 10 µmol/L) (groups 3 and 4) reduced late apoptosis significantly in exposed fibroblasts compared to exposed cells alone (group 2). The addition of vitamin E significantly reduced early apoptosis in lymphocytes (25 and 50 µmol/l) (groups 4 and 5) and fibroblasts (10 and 50 µmol/l) (groups 3 and 5) as well as late apoptosis in fibroblasts (10 and 25 µmol/l) (groups 3 and 4) in comparison to the respective group with exposure alone (group 2).
The authors conclude that vitamin C and vitamin E could protect fibroblasts and lymphocytes from apoptosis induced by a 50 Hz magnetic field and that this effect was stronger in fibroblasts.