Medical/biological study (experimental study)

Vitamin C and Vitamin E Protected B95-8 and Balb/c-3T3 Cells from Apoptosis Induced by Intermittent 50Hz ELF-EMF Radiation.

Published in: Iran J Public Health 2017; 46 (1): 23-34

Aim of study (acc. to author)

To examine the influence of a 50 Hz magnetic field on apoptosis in lymphocytes and fibroblasts as well as the possible protective effect of vitamin E (Vit E) and vitamin C (Vit C).
Background/further details: Lymphocytes and fibroblasts were used and respectively five groups were examined for every cell line and antioxidant: 1) control group without magnetic field exposure, 2) magnetic field exposure, 3) magnetic field exposure with 10 µmol/l Vit E (or 2 µmol/l Vit C), 4) magnetic field exposure with 25 µmol/l Vit E (or 10 µmol/l Vit C) and 5) magnetic field exposure with 50 µmol/l Vit E (or 100 µmol/l Vit C). The antioxidants were added six hours before exposure.



Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuously for 16 hours
Exposure 1
Main characteristics
Frequency 50 Hz
Exposure duration continuously for 16 hours
Exposure setup
Exposure source
Chamber 50 mm petri dishes were filled with 5 ml culture medium
Setup The two four-coil system (?) which generated the stable magnetic field was placed inside a µ-metal box; heating system kept the temperature at 37°C in both waveguides; CO2 was 5%
Measurand Value Type Method Mass Remarks
magnetic flux density 2.3 mT unspecified unspecified - -
Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated material:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In exposed fibroblasts (group 2), the percentage of apoptotic cells was higher compared to the respective control group (group 1). The addition of vitamin C (2 and 10 µmol/L) (groups 3 and 4) reduced late apoptosis significantly in exposed fibroblasts compared to exposed cells alone (group 2). The addition of vitamin E significantly reduced early apoptosis in lymphocytes (25 and 50 µmol/l) (groups 4 and 5) and fibroblasts (10 and 50 µmol/l) (groups 3 and 5) as well as late apoptosis in fibroblasts (10 and 25 µmol/l) (groups 3 and 4) in comparison to the respective group with exposure alone (group 2).
The authors conclude that vitamin C and vitamin E could protect fibroblasts and lymphocytes from apoptosis induced by a 50 Hz magnetic field and that this effect was stronger in fibroblasts.
Study character:

Study funded by

  • National Basic Research Program of China
  • Ministry of Science and Technology (MOST), China
  • National Key Technologies R&D Program, China
  • The State Key Laboratories Development Program, China

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