Medical/biological study (experimental study)

Vitamin C and Vitamin E Protected B95-8 and Balb/c-3T3 Cells from Apoptosis Induced by Intermittent 50Hz ELF-EMF Radiation med./bio.

By: Ding Z, Li J, Li F, Mephryar MM, Wu S, Zhang C, Zeng Y

Published in: Iran J Public Health 2017; 46 (1): 23-34

To examine the influence of a 50 Hz magnetic field on apoptosis in lymphocytes and fibroblasts as well as the possible protective effect of vitamin E (Vit E) and vitamin C (Vit C).

Lymphocytes and fibroblasts were used and respectively five groups were examined for every cell line and antioxidant: 1) control group without magnetic field exposure, 2) magnetic field exposure, 3) magnetic field exposure with 10 µmol/l Vit E (or 2 µmol/l Vit C), 4) magnetic field exposure with 25 µmol/l Vit E (or 10 µmol/l Vit C) and 5) magnetic field exposure with 50 µmol/l Vit E (or 100 µmol/l Vit C). The antioxidants were added six hours before exposure.

Exposure	Parameters
Exposure 1: 50 Hz Exposure duration: continuously for 16 hours	magnetic flux density: 2.3 mT unspecified

Exposure source	coil(s)
Chamber	50 mm petri dishes were filled with 5 ml culture medium
Setup	The two four-coil system (?) which generated the stable magnetic field was placed inside a µ-metal box; heating system kept the temperature at 37°C in both waveguides; CO₂ was 5%

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	2.3 mT	unspecified	unspecified	-	-

Exposed system:

Investigated system:

Time of investigation:

In exposed fibroblasts (group 2), the percentage of apoptotic cells was higher compared to the respective control group (group 1). The addition of vitamin C (2 and 10 µmol/L) (groups 3 and 4) reduced late apoptosis significantly in exposed fibroblasts compared to exposed cells alone (group 2). The addition of vitamin E significantly reduced early apoptosis in lymphocytes (25 and 50 µmol/l) (groups 4 and 5) and fibroblasts (10 and 50 µmol/l) (groups 3 and 5) as well as late apoptosis in fibroblasts (10 and 25 µmol/l) (groups 3 and 4) in comparison to the respective group with exposure alone (group 2).
The authors conclude that vitamin C and vitamin E could protect fibroblasts and lymphocytes from apoptosis induced by a 50 Hz magnetic field and that this effect was stronger in fibroblasts.

Study character:

Zeng Y et al. (2017): Effects of Single and Repeated Exposure to a 50-Hz 2-mT Electromagnetic Field on Primary Cultured Hippocampal Neurons
Pasi F et al. (2016): Effects of extremely low-frequency magnetotherapy on proliferation of human dermal fibroblasts
Feng B et al. (2016): Mitochondrial ROS Release and Subsequent Akt Activation Potentially Mediated the Anti-Apoptotic Effect of a 50-Hz Magnetic Field on FL Cells
Zhang H et al. (2016): Protective effect of procyanidins extracted from the lotus seedpod on immune function injury induced by extremely low frequency electromagnetic field
An GZ et al. (2015): Effects of long-term 50 Hz power-line frequency electromagnetic field on cell behavior in Balb/c 3T3 cells
Brisdelli F et al. (2014): ELF-MF attenuates quercetin-induced apoptosis in K562 cells through modulating the expression of Bcl-2 family proteins
Saha S et al. (2014): Increased apoptosis and DNA double-strand breaks in the embryonic mouse brain in response to very low-dose X-rays but not 50 Hz magnetic fields
Akdag MZ et al. (2013): Can safe and long-term exposure to extremely low frequency (50 Hz) magnetic fields affect apoptosis, reproduction, and oxidative stress?
Palumbo R et al. (2006): Effects on apoptosis and reactive oxygen species formation by Jurkat cells exposed to 50 Hz electromagnetic fields
De Nicola M et al. (2006): Magnetic fields protect from apoptosis via redox alteration