Study type: Medical/biological study (experimental study)

Effects on apoptosis and reactive oxygen species formation by Jurkat cells exposed to 50 Hz electromagnetic fields. med./bio.

Published in: Bioelectromagnetics 2006; 27 (2): 159-162

Aim of study (acc. to author)

To study the effect of 1 h intermittent exposure to 50 Hz electromagnetic field (power transmission line signal) on Jurkat cells by evaluating the reactive oxygen species production and apoptosis, both spontaneous and induced by the well kown pro-apoptotic agent, anti-Fas (50 ng/ml).

Background/further details

Jurkat cells were employed because it has been reported that treatment with anti-Fas resulted in proteolytic cleavage of precursors of caspase-3, thus triggering an apoptotic process.
Positive controls were provided by treating the culture for 1 h with 500 µM H2O2.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: intermittent, 5 min field on/10 min field off for 1 h

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration intermittent, 5 min field on/10 min field off for 1 h
Exposure setup
Exposure source
Setup Exposure system consisted of two identical apparatuses, each composed of four square coils placed horizontally inside a µ-metal shielding box. The coils were arranged in wound configuration for exposure or in counter wound configuration for sham exposure.The magnetic field direction was perpendicular to the surface of the culture plates.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT effective value unspecified - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data suggest that 1 h intermittent exposure does not affect reactive oxygen species formation, while a slight but statistically significant decrease of both spontaneous and anti-Fas-induced apoptosis was revealed.

Study character:

Study funded by

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