|Chamber||5,000 eggs (and later on worms) were cultivated and exposed in one plate|
|Setup||exposure system consisted of five parts including a power regulator, two Helmholtz coils, an incubator, a temperature recorder and a temperature control system; two vertical coils (150 turns of 1mm copper wire) were placed in the incubator and connected in parallel; three culture plates were put simultaneously in the center of the coils where the magnetic field was uniform; a silicone tube connected to a condenser was wound around the coils to keep the temperature at 20 °C (± 0.10 °C)|
|Sham exposure||A sham exposure was conducted.|
|Additional info||control samples were handled identically except for the magnetic field exposure|
|magnetic flux density||3 mT||-||measured||-||-|
Gene expression of fum-1 and mdh-2 was significantly decreased under magnetic field exposure when compared to the control group. This effect was verified for FUM-1 via Western blot. The levels of arachidonic acid and prostaglandin E2 were significantly increased in the exposed worms in comparison to the control ones. Additionally, the protein expression of the prostaglandin E2 synthase was significantly increased.
In exposed worms, the level of reactive oxygen species was significantly increased compared to control ones while the total antioxidant capacity was significantly decreased.
The authors conclude, that exposure to 50 Hz magnetic fields affects the tricarboxylic acid cycle and prostaglandin E2 synthesis in Caenorhabditis elegans and that these alterations are coupled to oxidative stress responses.