Study type: Medical/biological study (experimental study)

Exposure to 50 Hz electromagnetic fields enhances hair follicle regrowth in C57BL/6 mice. med./bio.

Published in: Exp Biol Med (Maywood) 2019; 244 (5): 389-394

Aim of study (acc. to author)

The effects of exposure of mice to a 50 Hz magnetic field on hair follicle regrowth should be investigated.

Background/further details

Mice were depilated at dorsal skin and divided at random into a control group and exposure group (sizes of groups not mentioned). After 3, 9 and 16 days of exposure, 3 animals per group were investigated, respectively. The experiment was repeated 3 times. (remark EMF-Portal: A total of 27 animals per experiment, which is mentioned in the article, is not comprehensible.)

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 30 minutes per day for up to 16 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 30 minutes per day for up to 16 days
Exposure setup
Exposure source
Setup exposure system was stored in a room at 25°C with 50 ± 10% humidity
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5 mT - - - -

Reference articles

  • Li X et al. (2012): Effects of 50 Hz pulsed electromagnetic fields on the growth and cell cycle arrest of mesenchymal stem cells: an in vitro study.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The histological analysis showed that hair follicle growth was increased in the exposure group compared to the control group at all time points. The hair lenght was significantly increased in the exposure group compared to the control group after 16 days. Moreover, immunohistochemical staining revealed increased cell proliferation in the outer root sheath and hair bulb in the exposure group compared to the control group at all time points where it was co-localized with epidermal stem cells. Keratinocyte growth factors were expressed in the inner root sheath in both groups, and in Western Blot, the exposure group showed a higher expression rate at all time points than the control group (significance unclear).
The authors conclude that exposure of mice to a 50 Hz magnetic field could promote hair follicle regrowth in association with stem cells proliferation and increased keratinocyte growth factor expression.

Study character:

Study funded by

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