Study type: Medical/biological study (experimental study)

Effects of 50 Hz electromagnetic fields on human epidermal stem cells cultured on collagen sponge scaffolds. med./bio.

Published in: Int J Radiat Biol 2012; 88 (7): 523-530

Aim of study (acc. to author)

To examine the effects of a magnetic field-exposure on the cell proliferation of epidermal stem cells on collagen sponge scaffolds.

Background/further details

Skin tissue engineering is a promising tool for the treatment of epidermal injuries (e.g. transplantation of skin after burning). The success depends on many variables, such as used cells, scaffold material and environmental aspects. To test whether magnetic field could promote the cell proliferation, human foreskin cells were exposed or sham exposed for 14 days. Cell morphology was examined after 2, 7, 10 and 14 days.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 30 min/day for 2, 7, 10, or 14 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 30 min/day for 2, 7, 10, or 14 days
Exposure setup
Exposure source
Setup pair of coils with a distance of 10 cm
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

Exposed cell cultures showed a significant increased number of cells after 7, 10 and 14 days of exposure compared to the control culture. Additionally, the exposed cells grew together and became flaky after 14 days, while the control cells grew as a mass.
The results suggest that magnetic fields could promote cell proliferation in human epidermal stem cells.

Study character:

Study funded by

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