Study type: Medical/biological study (experimental study)

Evaluation of the influence of in vivo exposure to extremely low-frequency magnetic fields on the plasma levels of pro-inflammatory cytokines in rats. med./bio.

Published in: Int J Radiat Biol 2018; 94 (10): 909-917

Aim of study (acc. to author)

The effects of exposure of rats to a 50 Hz magnetic field on the plasma concentration of pro-inflammatory cytokines and other hematological parameters should be investigated.

Background/further details

Rats were randomly divided into the following groups (n=6, respectively): 1) continuous exposure for 24 hours to a 7 mT magnetic field, 2) repetitive exposure for 1 hour/day for 7 days to a 7 mT magnetic field. For each exposure group, a separate sham exposure group was used. Moreover, there was a cage control.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 24 hours
Exposure 2: 50 Hz
Exposure duration: 1 hour/day for 7 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 24 hours
Exposure setup
Exposure source
Chamber plexiglas cage (one animal per cage, 6 cm x 11 cm x 21 cm)
Setup exposure was carried out in a solenoid (19 cm inner diameter and 21 cm in length); force lines were parallel to the long axis of the animals; distribution of the magnetic field was measured every 1 cm along the main axis of the solenoid and the non-homogeneity of the field within the area containing the animal’s cage was approximately 10%; temperature during the experiments (for all groups) was set to 24 ± 1°C
Sham exposure A sham exposure was conducted.
Additional info sham exposure apparatus had the same size as exposure system but lacking a coil
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 7 mT - measured - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 1 hour/day for 7 days
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 7 mT - measured - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Continuous exposure for 24 hours to the magnetic field (group 1) resulted in a significant increase of IL-1b, IL-6 and IL-2 levels in plasma as well as a significant increase in white blood cells, lymphocytes, red blood cells, hemoglobin and hematocrit levels compared to all other groups, including sham exposure.
The authors conclude that continuous exposure for 24 hours of rats to a 50 Hz magnetic field might influence the immune response, while repetitive exposure of 1 hour/day has no effect. Hence, exposure duration might be a critical factor for the immune reactivity.

Study character:

Study funded by

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