To study the effects of radiofrequency fields modulated in amplitude at an extremely low frequency on the transcription of the calcium-modulated ETS1 oncogene in hemopoietic and testicular cell types (exposure conditions were selected to interfere with calcium ion flow).
|Exposure duration||from 30 min to 24 h|
|Modulation frequency||16 Hz|
|Modulation depth||80 %|
An overexpression of the ETS1 mRNA in Jurkat T-lymphoblastoid and TM3 cell lines was observed. This effect was found only in the presence of the 16 Hz modulation, corresponding to the resonance frequency for calcium ion with a direct current magnetic field of 45.7 µT. The authors have also identified a putative candidate gene repressed after electromagnetic field exposure.
In conclusion, the experimental approach, based on transcriptional analysis, represents a reliable method for identification of electromagnetic field biological effects and ETS1 seems to be a specific gene target of electromagnetic field action in different cell types.