Study type: Medical/biological study (experimental study)

Ets1 oncogene induction by ELF-modulated 50 MHz radiofrequency electromagnetic field. med./bio.

Published in: Bioelectromagnetics 2000; 21 (1): 8-18

Aim of study (acc. to editor)

To study the effects of radiofrequency fields modulated in amplitude at an extremely low frequency on the transcription of the calcium-modulated ETS1 oncogene in hemopoietic and testicular cell types (exposure conditions were selected to interfere with calcium ion flow).

Background/further details

ETS1 is a member of a family of transcription factors involved in cell differentiation, tumorigenesis, and apoptosis.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 MHz
Modulation type: AM
Exposure duration: from 30 min to 24 h

Exposure 1

Main characteristics
Frequency 50 MHz
Type
Exposure duration from 30 min to 24 h
Modulation
Modulation type AM
Modulation frequency 16 Hz
Modulation depth 80 %
Exposure setup
Exposure source
Chamber incubator
Setup T5 flasks placed on the septum in one of the TEM cells
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 46 µT - measured - static MF at 60° inclination
magnetic flux density 200 nT - measured - MF parallel to the ground
electric field strength 60 V/m - measured - perpendicular to the earth`s MF

Reference articles

  • Blackman CF et al. (1985): Effects of ELF (1-120 Hz) and modulated (50 Hz) RF fields on the efflux of calcium ions from brain tissue in vitro.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

An overexpression of the ETS1 mRNA in Jurkat T-lymphoblastoid and TM3 cell lines was observed. This effect was found only in the presence of the 16 Hz modulation, corresponding to the resonance frequency for calcium ion with a direct current magnetic field of 45.7 µT. The authors have also identified a putative candidate gene repressed after electromagnetic field exposure.
In conclusion, the experimental approach, based on transcriptional analysis, represents a reliable method for identification of electromagnetic field biological effects and ETS1 seems to be a specific gene target of electromagnetic field action in different cell types.

Study character:

Study funded by

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