Study type: Medical/biological study (experimental study)

Pulsed electromagnetic field stimulation of MG63 osteoblast-like cells affects differentiation and local factor production. med./bio.

Published in: J Orthop Res 2000; 18 (4): 637-646

Aim of study (acc. to author)

To examine the response of osteoblast-like cells to a pulsed electromagnetic field signal used clinically and to determine if the signal modulates the production of autocrine factors associated with differentiation.



Exposure Parameters
Exposure 1: 15 Hz
Modulation type: pulsed
Exposure duration: 8 h/d for 1, 2 or 4 days

Exposure 1

Main characteristics
Frequency 15 Hz
Exposure duration 8 h/d for 1, 2 or 4 days
Modulation type pulsed
Rise time 200 µs
Fall time 25 µs
Additional info

5 ms bursts of 20 pulses

Exposure setup
Exposure source
Chamber Incubator with Plexiglas shelves.
Setup Culture placed between Helmholtz coils
Measurand Value Type Method Mass Remarks
magnetic flux density 1.8 mT effective value unspecified - increased from 0 T to 1.8 mT within 200 µs and decayed back to 0 T in 25 µs.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The results show that pulsed electromagnetic field stimulation affects the proliferation, differentiation, extracellular matrix synthesis, and local factor production of MG63 cells. Exposed cells exhibited a more differentiated phenotype, as evidenced by reduced cell proliferation and increased alkaline phosphatase activity. Pulsed electromagnetic field stimulation affected osteocalcin production, increases TGF-β1 levels and inhibits the synthesis of prostaglandin E2. Cell number and [3H]thymidine incorporation were reduced.

Study character:

Study funded by

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