Study type: Medical/biological study (experimental study)

Electromagnetic field change the expression of osteogenesis genes in murine bone marrow mesenchymal stem cells. med./bio.

Published in: J Huazhong Univ Sci Technolog Med Sci 2008; 28 (2): 152-155

Aim of study (acc. to author)

To study the effect of electromagnetic fields on osteogenesis of bone marrow mesenchymal stem cells.

Background/further details

The bone marrow mesenchymal stem cells of Sprague-Dawley rats were isolated and cultured in vitro.
Osteogenesis microarray was used to detect the gene expression of genes which only occur during the process of cell differentiation. On the basis of the microarray results, RT-PCR was used to identify the typical genes.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 15 Hz
Exposure duration: continuous for 8 h/day on 2 days

Exposure 1

Main characteristics
Frequency 15 Hz
Type
Waveform
Exposure duration continuous for 8 h/day on 2 days
Exposure setup
Exposure source
  • not specified
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In the exposed cells 19 differently expressed genes were found compared to the control group. There were six up-regulated genes and 13 down-regulated genes in the exposed group. Semiquantitative RT-PCR confirmed that the expressions of the genes Bmp1, Bmp7 of the exposed group were up-regulated and those of EGF, EGFR were down-regulated.
It was suggested that the gene expression profiles of osteogenesis of the bone marrow mesenchymal stem cells were changed after electromagnetic field treatment.

Study character:

Study funded by

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