A culture dish in which the medium stood 8 mm high was placed on two slits in the top wall of the waveguide. The slits were optimized with a pinched shape to increase the effective exposure area and enhance the uniformity of the electric field and the SAR distribution in the medium. One end of the waveguide was terminated with a short-circuiting plate to generate standing waves.
The temperatures of the medium at the maximum SAR position measured using a fibre optic thermometer rose to 39.1 °C, 41.0 °C, and 44.1 °C after exposure to 50, 100, and 200 W/kg, respectively, for 2 h at an ambient temperature of 37 °C. Cells for raised temperature control were incubated at 39, 40, 41, 42, 43, and 44 °C in a conventional incubator for the same time periods.
Rectangular culture dishes divided into four compartments were used. Compartments 2 and 4 provided 80 and 85% of the maximum SAR value obtained in compartment 3, whereas in compartment 1, the SAR was less than 1% of the maximum value.
Measurement and calculation details
The incident and reflected powers were monitored by powersensors. SAR distribution was calculated using the FDTD method and confirmed experimentally by measuring the temperature increase in the medium over a short period following acute exposure using a DASY 3 positioning system with a thermistor probe.
Ministry of Internal Affairs and Communications, Japan
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