Study type: Medical/biological study (experimental study)

Decreased DNA repair rates and protection from heat induced apoptosis mediated by electromagnetic field exposure. med./bio.

Published in: Bioelectromagnetics 2002; 23 (2): 106-112

Aim of study (acc. to author)

To investigate the effects of extremely low frequency electromagnetic field on DNA repair and apoptosis in human cell lines in vitro.

Background/further details

DNA damage was induced by H2O2, apoptosis by heat shock (43°) and both investigated in three different human cancer cell lines.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: 4, 12 and 24 h

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration 4, 12 and 24 h
Exposure setup
Exposure source
Exposure room 25ml cell culture flasks
Setup inner diameter of the coil 14 cm; height 10 cm; flasks with cell cultures placed in the center of the coil perpendicular to MF
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 150 µT peak value calculated - +/- 0.02 mT
power 120 W - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

No significant difference was observed in the cell proliferation between electromagnetic field exposed and sham exposed cells.
The exposure to extremely low frequency electromagnetic field revealed time dependent protection from heat induced apoptosis in all three cell lines.
The DNA repair rates for electromagnetic field exposed HL-60 and HL-60R cell lines were significantly decreased. No significant difference in DNA repair were observed in electromagnetic field exposed Raji cells compared to sham exposed cells.

Study character:

Study funded by

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