Repetitive exposure to a 60-Hz time-varying magnetic field induces DNA double-strand breaks and apoptosis in human cells med./bio.

Aim of study (acc. to author)

Endpoint

• genotoxicity/mutation: DNA damage
• cell viability/cell division/proliferation: apoptosis

Exposure

• • 50/60 Hz
  • magnetic field

Extended view Compact view

Methods Endpoint/measurement parameters/methodology

• genotoxicity/mutation: DNA damage (double-strand break; formation of gamma-H2AX foci (fluorescence microscopy) and phosphorylation of gamma-H2AX (Western blot)) and DNA damage checkpoint pathway (phospho-checkpoint kinase 2 expression; Western blot)
• molecular biosynthesis: expression of stress-activated protein kinases ((phospho-) p38 mitogen activated protein kinase and (pospho-) JNK mitogen-activated protein kinase; Western blot)
• cell viability/cell division/proliferation: apoptosis and apoptosis-related pathways (expression of caspase 3, cleaved caspase-3 and PARP; Western blot), cell proliferation/cell viability (MTT assay)

Main outcome of study (acc. to author)

Study funded by

• Korea Research Institute of Chemical Technology (KRICT)
• Ministry of Knowledge Economy (MKE), Korea
• Yonsei University Research Fund, Korea

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