The aim of the study was to investigate both the possibility that 1H-NMR can be used to reveal even the most subtle damage induced by extremely low frequency fields as well as to demonstrate possible signs of apoptosis.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
continuous for 2 h
|
|
|
Exposure 2:
50 Hz
Exposure duration:
continuous for 2 h
|
|
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | continuous for 2 h |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 1 mT | effective value | measured | - | ± 3% measurement accuracy |
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | continuous for 2 h |
| Exposure source |
|
|---|
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 5 mT | effective value | measured | - | ± 3% measurement accuracy |
The variations in numerous metabolites found with 1H-NMR reveal apoptosis-like behavior in response of cells to extremely low frequency fields. 1H-NMR can be extremely useful in investigating the effects of extremely low frequency fields on cells, particularly in better comprehending the dynamics of the early stages of apoptosis. The variations in metabolites which suggest apoptosis-like behavior occur when the cells are not identifiable as apoptotic by more traditional techniques.
Whether or not magnetic field-exposed cells will die or whether the apoptotic process will be reversed is not known. Nonetheless, it is apparent that extremely low frequency fields affect cells in very important ways. Further studies are necessary in order to better understand these effects.
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