Study type: Medical/biological study (experimental study)

A 700 MHz 1H-NMR study reveals apoptosis-like behavior in human K562 erythroleukemic cells exposed to a 50 Hz sinusoidal magnetic field. med./bio.

Published in: Int J Radiat Biol 2005; 81 (2): 97-113

Aim of study (acc. to author)

The aim of the study was to investigate both the possibility that 1H-NMR can be used to reveal even the most subtle damage induced by extremely low frequency fields as well as to demonstrate possible signs of apoptosis.

Background/further details

The spectra of whole cells as well as of the perchlorid extracts of these cells were evaluated.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 2 h
Exposure 2: 50 Hz
Exposure duration: continuous for 2 h

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 2 h
Exposure setup
Exposure source
Setup 75 cm² tissue culture flasks containing K562 cells were placed in the center of the coils. The setup was maintained at 37°C. The magnetic field was parallel to the cells.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT effective value measured - ± 3% measurement accuracy

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 2 h
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5 mT effective value measured - ± 3% measurement accuracy

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The variations in numerous metabolites found with 1H-NMR reveal apoptosis-like behavior in response of cells to extremely low frequency fields. 1H-NMR can be extremely useful in investigating the effects of extremely low frequency fields on cells, particularly in better comprehending the dynamics of the early stages of apoptosis. The variations in metabolites which suggest apoptosis-like behavior occur when the cells are not identifiable as apoptotic by more traditional techniques.
Whether or not magnetic field-exposed cells will die or whether the apoptotic process will be reversed is not known. Nonetheless, it is apparent that extremely low frequency fields affect cells in very important ways. Further studies are necessary in order to better understand these effects.

Study character:

Study funded by

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