Study type: Medical/biological study (experimental study)

Effects of pulsed electric fields on DNA of human lymphocytes. med./bio.

Published in: Cell Biol Toxicol 2006; 22 (6): 409-415

Aim of study (acc. to author)

To study the effects of pulsed low frequency electric fields (50 Hz) on DNA of human lymphocytes of 40 men.

Background/further details

The influence of additional external factors (synergistic effects), such as hydrogen peroxide (0. 50, 100, or 150 µmol/l) and gamma radiation (0, 0.8, 2.5, or 4.2 Gy) was also determined.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 10–50 Hz
Modulation type: pulsed
Exposure duration: continuous for 120 min

Exposure 1

Main characteristics
Frequency 10–50 Hz
Type
Waveform
Exposure duration continuous for 120 min
Modulation
Modulation type pulsed
Packets per second 10
Repetition frequency 50 Hz
Additional info

"decreasing sinusoidally bipolar pulses of a positive half period, with pulse frequency of 50 Hz and carrier frequency 10 Hz [Panagopoulos et al., 2000]"

Exposure setup
Exposure source
Chamber Thawed lymphocytes suspended in ice cold PBS were exposed in microcentrifuge tubes at 5 ± 0.5°C.
Setup Using network electricity, short-duration pulses were transformed into high voltage.
Additional info Lymphocytes were also exposed to 0, 50, 100, or 150 µmol/l H2O2 or to 0, 0.8, 2.5, or 4.2 Gy γ radiation from a 60Co source at a dose rate of 3.99 Gy/min after being treated or not with the pulsed electric field.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 400 kV/m unspecified unspecified - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

A significant increased DNA damage was found after exposure to the electric fields, compared to the controls. After 2 h incubation at 37°C, a proportion of damage was repaired.
Hydrogen peroxide and gamma radiation increased the DNA damage of lymphocytes exposed to pulsed electric fields according to the dose used, while the amount of the DNA repair was proportional to the damage.

Study character:

Study funded by

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