Study type: Medical/biological study (experimental study)

Functional alterations in immature cultured rat hippocampal neurons after sustained exposure to static magnetic fields. med./bio.

Published in: J Neurosci Res 2004; 75 (2): 230-240

Aim of study (acc. to author)

To investigate the effects of exposure to static magnetic fields on maturation and differentation in rat hippocampal neuron cells in vitro.

Background/further details

Hippocampal neurons and astrocytes, as well as astrocytes from brain neocortex were obtained from 18 and 19 day-old embryonic rats.

Endpoint

Exposure

Exposure Parameters
Exposure 1:
Exposure duration: continuous up to 9 days

Exposure 1

Main characteristics
Frequency
Type
Exposure duration continuous up to 9 days
Exposure setup
Exposure source
Chamber CO2 incubator at 37°C.
Setup magnets placed on both sides of the culture dishes which were placed 10 cm away from each other
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 100 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Sustained exposure to static magnetic fields leads to a significantly decrease of expression of MPA-2 (microtubule-associated protein 2) and NeuN (neuronal nuclei). The expression of GAP-3 (growth-associated protein 3) was not markedly affected. In hippocampal neurons expression of GFAP (glial fibrillary acidic protein) was significantly increased. However, this was not found in astrocytes from hippocampus and neocortex.
Cell viability of investigated cells was not significantly altered by magnetic field exposure. In hippocampal neurons a marked mRNA expression of NMDA (N-methyl-D-aspartate) receptor subunits was seen. In addition, an increase of intracellular free calcium due to the ability of NMDA was observed. Finally a significant decrease in mRNA expression for a transcription factor (ALF 1) was found.
The data of this study suggest that static magnetic fields may affect cultured embryonic rat hippocampal neurons. This might be through modulated expression of particular NMDA rezeptor units and altered intracellular free calcium levels.

Study character:

Study funded by

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