|magnetic flux density||100 mT||-||measured||-||-|
Sustained exposure to static magnetic fields leads to a significantly decrease of expression of MPA-2 (microtubule-associated protein 2) and NeuN (neuronal nuclei). The expression of GAP-3 (growth-associated protein 3) was not markedly affected. In hippocampal neurons expression of GFAP (glial fibrillary acidic protein) was significantly increased. However, this was not found in astrocytes from hippocampus and neocortex.
Cell viability of investigated cells was not significantly altered by magnetic field exposure. In hippocampal neurons a marked mRNA expression of NMDA (N-methyl-D-aspartate) receptor subunits was seen. In addition, an increase of intracellular free calcium due to the ability of NMDA was observed. Finally a significant decrease in mRNA expression for a transcription factor (ALF 1) was found.
The data of this study suggest that static magnetic fields may affect cultured embryonic rat hippocampal neurons. This might be through modulated expression of particular NMDA rezeptor units and altered intracellular free calcium levels.