Study type: Medical/biological study (experimental study)

Developmental effects of low frequency magnetic fields on P19-derived neuronal cells. med./bio.

Published in: No authors listed, 2009 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, Minneapolis, MN, USA IEEE, 2009: 5942-5945, ISBN 9781424432967

Aim of study (acc. to author)

The aim of this study was to investigate the effect of extremely low frequency magnetic fields (1 mT and 10 mT) on the neuronal differentiation process (cell morphology and electrical activity) of an embryonal carcinoma stem cell model.

Background/further details

The extremely low frequency magnetic field exposure was applied to embryoid bodies (aggregates of cells derived from embryonic stem cells) formed at induction of neuronal cell differentiation process and the effects of exposure were evaluated after neuronal differentiation.
The spontaneous electrical activities of neuronal cells are generated during the differentiation process of neuronal networks, and these activities show clearly different behavior according to differentiation periods.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: during cell differentiation

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration during cell differentiation
Exposure setup
Exposure source
Setup the Helmholtz coils was set in a CO2 incubator with constant temperature
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - measured - -
magnetic flux density 10 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data showed that the size of embryoid bodies exposed to 10 mT extremely low frequency magnetic fields was significantly decreased compared to non-exposed embryoid bodies. The cell differentiation rate of neuronal cells and functional neuronal network activities were also significantly modulated by exposure to 10 mT magnetic field: The percentage of MAP2-positive cells was increased and GFAP positive cells was decreased at 14 and 21 days after plating. Additionally, the spike frequencies and the active electrodes (i.e. electrodes that detected action potentials) of the spontaneous electrical activity in this period were also increased. However, these effects were not found in 1 mT exposed cells.
In conclusion, exposure of embryoid bodies at the induction of differentiation period to 10 mT extremely low frequency magnetic fields had a functional influence on the neuronal differentiation process.

Study character:

Study funded by

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