Study type: Medical/biological study (experimental study)

Activator protein-1 complex expressed by magnetism in cultured rat hippocampal neurons. med./bio.

Published in: Biochem Biophys Res Commun 2002; 292 (1): 200-207

Aim of study (acc. to author)

To study the influence of static magnetic field on DNA binding activity and expression of nuclear AP-1 (activator protein-1) complex in immature and mature cultured rat cortical and hippocampal neurons as a guidepost for possible long-lasting functional alterations by magnetism in the brain.

Background/further details

c-Fos protein family is a partner of c-Jun protein family members to compose the nuclear transcription factor AP-1.

Endpoint

Exposure

Exposure Parameters
Exposure 1:
Exposure duration: continuous for 15 min

Exposure 1

Main characteristics
Frequency
Exposure duration continuous for 15 min
Exposure setup
Exposure source
Setup Magnets were placed at both sides of the culture dishes in a CO2 incubator maintained at 37°C. Sham exposure group was subjected to the same conditions and procedure as the exposure group.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 100 mT - unspecified - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Brief exposure for 15 min to static magnetic field (100 mT) resulted in marked but transient expression of AP-1 complex with DNA binding activity in immature cultured hippocampal neurons. The data revealed that brief exposure to static magnetic field increased AP-1 DNA binding through expression of Fra-2, c-Jun, and Jun-D proteins in immature cultured hippocampal neurons.
Transient magnetic stimulation would induce long-lasting alterations of a variety of cellular functions through modulation of de novo synthesis of particular inducibel target proteins at the level of gene transcription by AP-1 complex in the nucleus of immature cultured hippocampal neurons. For example, prior brief exposure to magnetism could result in desensitization of NMDA (N-methyl-D-aspartate) receptor channels with regard to increase in intracellular free Ca2+ concentration (as determined by fluorescence imaging) and activator protein-1 binding in immature neurons.

Study character:

Study funded by

Related articles