Study type: Medical/biological study (experimental study)

Investigation of protein expression in magnetic field-treated human glioma cells. med./bio.

Published in: Bioelectromagnetics 2007; 28 (7): 546-552

Aim of study (acc. to author)

This study was performed to investigate possible effects of low frequency magnetic field on protein expression in human glial cells in vitro.

Background/further details

The cells were exposed to magnetic field plus/without epidermal growth factor (EGF, 10 ng/ml). The experiments were replicated twice.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: 3 h continuous

General information

cells were treated in four groups: i) sham ii) exposure to 1.2 µT iii) sham + EGF (10 mg/ml) iv) exposure to 1.2 µT + EGF

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Exposure duration 3 h continuous
Exposure setup
Exposure source
Setup two concentric Helmholtz coils systems with the inner coil for exposure and the outer coil to limit stray fields
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1.2 µT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The mean abundance of 10 identified proteins was altered following magnetic field exposure (three proteins increased, seven proteins decreased).
The co-exposure to epidermal growth factor increased the expression of two proteins and decreased the expression of four proteins.
The results indicate that the analysis of differentially expressed proteins in the human glial cells (SF767) may serve as markers for biological changes caused by the exposure to magnetic fields.

Study character:

Study funded by

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