Study type: Medical/biological study (experimental study)

Effects of 60 Hz electromagnetic field exposure on testicular germ cell apoptosis in mice. med./bio.

Published in: Asian J Androl 2004; 6 (1): 29-34

Aim of study (acc. to author)

The effects of exposure of mice to a 60 Hz magnetic field on apoptosis in the testes should be investigated.

Background/further details

15 mice were randomly divided into 3 groups (n=5 each): 1) exposure to a magnetic field with 0.1 mT, 2) exposure to a magnetic field with 0.5 mT and 3) sham exposure (control group). All tests were repeated thrice.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous for 24 hours/day for 8 weeks
Exposure 2: 60 Hz
Exposure duration: continuous for 24 hours/day for 8 weeks

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Exposure duration continuous for 24 hours/day for 8 weeks
Exposure setup
Exposure source
Chamber non-metallic polycarbonate cages
Setup field was generated by three parallel rectangular coils in acryl frame; mice were placed in the center of the exposure chamber in cages fitted with non-mettallic water bottles and placed on trays of the exposure device; temperature was maintained at 19-22°C and humidity was kept between 30-70 %
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.1 mT - measured - ± <5%

Exposure 2

Main characteristics
Frequency 60 Hz
Type
Exposure duration continuous for 24 hours/day for 8 weeks
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.5 mT - measured - ± <5%

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated material:
Investigated organ system:
Time of investigation:
  • before exposure
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

No effects of the magnetic field on body or testes weight were found.
Johnson's biopsy score for testicular histology was significantly lower in both exposure groups compared to the control group.
Apoptosis in general and late apoptosis in special were significantly increased in both exposure groups compared to the control group. Apoptotic cells were mainly spermatogonia.
The authors conclude, that exposure of mice to a 60 Hz magnetic field could induce apoptosis in the testes.

Study character:

Study funded by

Comments on this article

Related articles