The effects of exposure of mice to a 60 Hz magnetic field on apoptosis in the testes should be investigated.
15 mice were randomly divided into 3 groups (n=5 each): 1) exposure to a magnetic field with 0.1 mT, 2) exposure to a magnetic field with 0.5 mT and 3) sham exposure (control group). All tests were repeated thrice.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
60 Hz
Exposure duration:
continuous for 24 hours/day for 8 weeks
|
|
|
Exposure 2:
60 Hz
Exposure duration:
continuous for 24 hours/day for 8 weeks
|
|
| Frequency | 60 Hz |
|---|---|
| Type | |
| Exposure duration | continuous for 24 hours/day for 8 weeks |
| Exposure source |
|
|---|---|
| Chamber | non-metallic polycarbonate cages |
| Setup | field was generated by three parallel rectangular coils in acryl frame; mice were placed in the center of the exposure chamber in cages fitted with non-mettallic water bottles and placed on trays of the exposure device; temperature was maintained at 19-22°C and humidity was kept between 30-70 % |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 0.1 mT | - | measured | - | ± <5% |
| Frequency | 60 Hz |
|---|---|
| Type | |
| Exposure duration | continuous for 24 hours/day for 8 weeks |
| Exposure source |
|
|---|
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 0.5 mT | - | measured | - | ± <5% |
No effects of the magnetic field on body or testes weight were found.
Johnson's biopsy score for testicular histology was significantly lower in both exposure groups compared to the control group.
Apoptosis in general and late apoptosis in special were significantly increased in both exposure groups compared to the control group. Apoptotic cells were mainly spermatogonia.
The authors conclude, that exposure of mice to a 60 Hz magnetic field could induce apoptosis in the testes.
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