|Exposure room||rats were placed in a cylindrical compartment made of polyvinyl chloride with openings for air intake, centrally located in the Helmholtz coil|
|Setup||pairs of Helmholtz coils; each coil was made of wire copper coated with vinyl, 154 turns, 38 cm diameter and 2.1 Ohm electrical impedance; coils were connected in parallel to a voltage generator, producing a uniform magnetic field; field lines crossed the animals transversely|
|Sham exposure||A sham exposure was conducted.|
|magnetic flux density||1 mT||-||measured||-||± 0.1 mT|
Plasma testosterone concentration was not changed by the magnetic field exposure.
After 30 and 60 days of exposure, the weight of the testes was significantly decreased compared to the sham exposure, while the gonadosomatic index was only significantly decreased after 60 days of exposure.
After 15 days of exposure, the decrease in the volume of the seminiferous tubules lumen was the only significantly changed parameter in comparison to the sham exposure. However, after 30 and 60 days several changes regarding the volume of the testes components, the cell ultrastructure and histomorphometrical parameters occurred in the exposure group when compared to the testes of sham exposed rats. Summarized, rats from the sham exposure group had a reestablished and active spermatogenesis 60 days after the heat shock procedure, while exposed animals still showed serious testicular degeneration.
The authors conclude that extremely low frequency magnetic fields could inhibit the spermatogenesis recovery after testicular degeneration induced by heat in rats.