Study type: Medical/biological study (experimental study)

In vitro study of effects of ELF-EMF on testicular tissues of roe deer (Capreolus capreolus) - FTIR and FT-Raman spectroscopic investigation. med./bio.

Published in: Anim Reprod Sci 2020; 213: 106258

Aim of study (acc. to author)

The effects of exposure to extremely low frequency magnetic fields testicular tissues of roe deer should be investigated.

Background/further details

Testicular tissue samples were divided into the following groups: 1) exposure to a magnetic field of 50 Hz for 2 hours, 2) 120 Hz for 2 hours, 3) control group for 2 hours, 4) exposure to a magnetic field of 50 Hz for 4 hours, 5) 120 Hz for 4 hours, 6) control group for 4 hours.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 2 and 4 hours
Exposure 2: 120 Hz
Exposure duration: 2 and 4 hours

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 2 and 4 hours
Exposure setup
Exposure source
  • Astar generator
Chamber 24-well plates were placed in a water bath, covered with a plastic plate, and flat applicators of generator were placed above the plates
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 8 mT - measured - -

Exposure 2

Main characteristics
Frequency 120 Hz
Type
Waveform
Exposure duration 2 and 4 hours
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 8 mT - measured - -

Reference articles

  • Koziorowska A et al. (2017): The impact of electromagnetic fields with frequency of 50 Hz on metabolic activity of cells in vitro.
  • Koziorowska A et al. (2016): The impact of electromagnetic field at a frequency of 50 Hz and a magnetic induction of 2.5 mT on viability of pineal cells in vitro.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Comparing the spectra from the exposure groups (groups 1, 2, 4 and 5) with the respective control groups (groups 3 and 6), there were several differences found in peaks representing lipids, proteins, carbohydrates, DNA and phospholipids. These peaks were partially shifted and their heights were different. A PCA analysis showed that the spectra of exposure groups were significantly different from their respective control groups, indicating differences in the chemical composition of testicular tissue.
The authors conclude that exposure to extremely low frequency magnetic fields might affect the testicular tissues of roe deer.

Study character:

Study funded by

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