To study the effect of electromagnetic field exposure and rosmarinic acid administration on Sertoli cells.
40 rats were divided into 4 groups (each group n=10): 1) control group, 2) rosmarinic acid administration alone (5 mg/rat or kg body weight (varying information) daily for 6 weeks), 3) electromagnetic field exposure, 4) electromagnetic field exposure + rosmarinic acid administration.
Exposure duration: 42 days (or 4 weeks; varying information)
|Exposure duration||42 days (or 4 weeks; varying information)|
|Setup||two copper coils placed one above the other in a distance of 50 cm; a cylindrical wooden vessel was placed between the coils (exposure area), the interior of which contained a chamber for holding the cage with the animals; 4 cages at a time were placed within the chamber, with ten rats per cage (remark EMF-Portal: make no sense in view of the group description?!)|
|magnetic flux density||8 mT||-||-||-||-|
Malondialdehyde level significantly decreased in the group that received rosmarinic acid alone (group 2) in comparison to the control group and increased in the exposure group (3) compared to the control group. Sertoli cells apoptosis was increased in all groups in comparison to the control group, but only significant in the exposure groups 3 and 4. Level of testosterone and total antioxidant capacity significantly increased in the group (2) that received rosmarinic acid alone compared to the control group. In contrast, testosterone level significantly decreased in the exposure group (3) compared to the control group.
The authors conclude that rosmarinic acid as food additive could be effective for supporting people living under electromagnetic field environmental pollution.