In a first phase of this investigation, a validation of the elevated plus-maze apparatus was performed in rats by testing anxiety response at various ambient light intensities (200, 30, 10 and 2.5 lux), as well as the effects of diazepam treatment (0.5 and 1.0 mg/kg, at 30 lux). Anxiety responses were revealed to decrease with decreasing light intensity and to be attenuated by diazepam treatment. Subsequently, a separate set of animals was exposed to 2.45 GHz electromagnetic fields for 45 min (at light intensity of 2.5 lux and 30 lux) to assess whether electromagnetic field exposure altered anxiety responses in the same apparatus.
Modulation type: pulsed
Exposure duration: continuous for 45 min
|Exposure duration||continuous for 45 min|
|Pulse width||2 µs|
|Repetition frequency||500 Hz|
|Exposure room||Transparent cylindrical plastic chamber/20 cm long, 17.5 cm in diameter, 13.5 cm high.|
|Setup||Each rats was placed in a cylindrical chamber located in the center of the waveguide.|
|Sham exposure||A sham exposure was conducted.|
Whatever light intensity was used, electromagnetic field irradiation failed to induce any significant effect on anxiety responses in the plus maze. The present experiment shows that exposure to electromagnetic fields does not alter anxiety responses assessed in the elevated plus maze.