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Thirty male rats were used. Behavioral changes were examined in the morning of the 21th, 23th, and 25th exposure day. Ratsanxiety-related behaviors were examined in the open field test, elevated plus maze, and the light/dark box during five minutes observations. The open field was a black circular area (diameter: 160 cm, height: 50 cm) with two concentric circles lines on the floor. The elevated plus maze consisted of two opposite open arms, an open platform in the center, and two opposite closed arms. To avoid fear rats tend to stay close to the sidewall in the open field and to the closed arms in the elevated plus maze. Grooming is interpreted as a stress coping and de-arousalbehavior response to increased anxiety level due to novel environment situations in the tests. The light/dark box was constructed from two equally sized boxes which were connected by an opening. In the dark box, there was no appreciable illumination. The light box was white and provided illumination at a level of 100 lux.
cognitive/behavioral endpoints: Anxiety-related behaviors (open field test: recording of traveled distance, time spent in outer and inner ring, grooming, defecation, rearing; elevated plus maze: spent time/number of entries in the open/closed arm, time spent in the central part, rearing in the open arms; light/dark box: recording of time spent in the light box, transitions between two boxes, latency to the dark box, rearing in the light box)
Rostami A et al.
Effects of 3 Hz and 60 Hz Extremely Low Frequency Electromagnetic Fields on Anxiety-Like Behaviors, Memory Retention of Passive Avoidance and Electrophysiological Properties of Male Rats.
Salunke BP et al.
Experimental evidence for involvement of nitric oxide in low frequency magnetic field induced obsessive compulsive disorder-like behavior.
Alsaeed I et al.
Autism-relevant social abnormalities in mice exposed perinatally to extremely low frequency electromagnetic fields.
Kitaoka K et al.
Chronic exposure to an extremely low-frequency magnetic field induces depression-like behavior and corticosterone secretion without enhancement of the hypothalamic-pituitary-adrenal axis in mice.