Murine cells were exposed to radiofrequency irradiation from a time division multiple access (TDMA) mobile phone operating at 835 MHz frequency to determine the effect of irradiation on ornithine decarboxylase (ODC) activity in cultured cells.
Modulation type: pulsed
Exposure duration: continuous for 8 h
|Exposure duration||continuous for 8 h|
|Chamber||The Crawford cell and a sham-exposure chamber with similar dimensions were placed in the same water-jacketed incubator maintained at 37°C.|
|Setup||Four 25-cm² flasks were placed in each exposure chamber with two flasks on each shelf. Effects of different temperatures in the absence of an RF field were examined in two satellite chambers (Plexiglas boxes receiving a controlled air flow from the central incubator).|
|Additional info||Though the exposure system produced a non-uniform spatial distribution of the absorbed power it was not replaced by a better one because the aim of the study was to reproduce the results obtained in the reference article. In addition, the longer exposure time chosen was expected to result in a more homogeneous temperature distribution.|
No statistically significant difference in ornithine decarboxylase activity was found between exposed and sham-exposed cells at non-thermal specific absorption rates (SARs). At SARs which resulted in measurable heating of the medium, a dose-dependent decrease in enzymatic activity was observed. It was shown to be consistent with a comparable decrease caused by non-radiofrequency irradiation heating. Thus the authors observed only the well-known enzyme inhibition due to heating, rather than the previously reported enhancement attributed to radiofrequency exposure (e.g. publication 3382 and publication 9896).