Study type: Medical/biological study (experimental study)

Radiofrequency radiation does not significantly affect ornithine decarboxylase activity, proliferation, or caspase-3 activity of fibroblasts in different physiological conditions. med./bio.

Published in: Int J Radiat Biol 2008; 84 (9): 727-733

Aim of study (acc. to author)

To test the hypothesis that variations in the physiological state of cells explain inconsistent results from in vitro studies on biological effects of radiofrequency irradiation.

Background/further details

Different experimental conditios were performed: 1) control cells, 2) radiofrequency exposed cells, 3) activated cells, 4) deprivated cells, 5) activated cells and radiofrequency exposure, and 6) deprivated cells and radiofrequency exposure. Activation of the cells was produced with fresh cell culture medium added prior to exposure. Cells were stressed with serum deprivation for 20 h before exposure.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 872 MHz
Modulation type: CW
Exposure duration: 1 h and 24 h for ODC measurement, 24 h for proliferation measurement, 1 h for caspase-3 activity measurement
Exposure 2: 872 MHz
Modulation type: pulsed
Exposure duration: 1 h and 24 h for ODC measurement, 24 h for proliferation measurement, 1 h for caspase-3 activity measurement

Exposure 1

Main characteristics
Frequency 872 MHz
Type
Exposure duration 1 h and 24 h for ODC measurement, 24 h for proliferation measurement, 1 h for caspase-3 activity measurement
Modulation
Modulation type CW
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 5 W/kg - - - -

Exposure 2

Main characteristics
Frequency 872 MHz
Type
Exposure duration 1 h and 24 h for ODC measurement, 24 h for proliferation measurement, 1 h for caspase-3 activity measurement
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 5 W/kg - - - -

Reference articles

  • Höytö A et al. (2006): Modest increase in temperature affects ODC activity in L929 cells: Low-level radiofrequency radiation does not.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

This study did not provide evidence of effects on ornithine decarboxylase activity, proliferation or caspase-3 activity in fibroblast cells exposed to 872 MHz radiofrequency irradiation at 5 W/kg. The results were similar for pulse-modulated and continuous wave irradiation. No statistically significant support was found for the hypothesis that altered physiological states (stimulation or stress) would alter cellular responses to radiofrequency radiation.

Study character:

Study funded by

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