Study type: Medical/biological study (experimental study)

ELF electromagnetic fields increase hydrogen peroxide (H2O2)-induced mutations in pTN89 plasmids. med./bio.

Published in: Mutat Res Genet Toxicol Environ Mutagen 2004; 560 (1): 27-32

Aim of study (acc. to author)

This in vitro study was performed to compare the mutagenic effects of hydrogen peroxide (H2O2) in the presence and absence of an extremely low frequency magnetic field.

Background/further details

To estimate the mutagenic effect pTN89 plasmids were used. Mutations were detected in the supF gene carried by these plasmids in E. coli. This system can find out a mutation at the single base level in the supF gene. The plasmid DNAs were treated with/without extremley low frequency electromagnetic field and than transformed in E. coli. The E. coli strain used is resitant to specific antibiotics if it contains a mutant supF gene forming white colonies on culture medium containing this antibiotics, whereas cells carrying an active supF gene do not produce colonies on such culture media.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous for 4 h

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Exposure duration continuous for 4 h
Exposure setup
Exposure source
Chamber CO2 incubator with a built in coils system kept at 37°C. The inside and outside of the incubators were shielded by silicon steel and Permally C respectively.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5 mT unspecified unspecified - -

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The exposure to extremely low frequency electromagnetic field alone did not show any mutation. The mutation frequency for plasmids treated with H2O2 and electromagnetic field was about 2.5-fold higher than that for treatment with H2O2 alone.
This indicates that the extremely low frequency electromagnetic field may potentiate H2O2-induced mutation.
The sequence analysis of the supF mutant plasmids revealed no difference in the mutation spectrum between the groups.

Study character:

Study funded by

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