Medical/biological study (experimental study)

Low dose magnetic fields do not cause oxidative DNA damage in human placental cotyledons in vitro. med./bio.

By: Lopucki M, Schmerold I, Dadak A, Wiktor H, Niedermuller H, Kankofer M

Published in: Virchows Arch 2005; 446 (6): 634-639

Aim of study (acc. to author)

This in vitro study was performed in order to gain an insight into potential effects of low intensity magnetic fields in human placental tissue.

Background/further details

8-hydroxy-2-deoxyguanosine was taken as marker of oxidative DNA damage.
31 placentas were used.

Endpoint

genotoxicity/mutation: oxidative DNA damage

Exposure

- 50/60 Hz
- magnetic field

Exposure	Parameters
Exposure 1: 50 Hz Exposure duration: continuous for 3 h	magnetic flux density: 2 mT unspecified magnetic flux density: 5 mT unspecified

Exposure 1

Main characteristics

Frequency	50 Hz
Type	magnetic field
Waveform	sinusoidal
Exposure duration	continuous for 3 h

Exposure setup

Exposure source	coil(s) electromagnet
Setup	electromagnet constructed by wounding a copper wire on a ferromagnetic core of 20 mm diameter; the magnetic field was uniformly distributed over the surface of 17.6 cm², ensuring exposure to the whole cotyledons
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	2 mT	unspecified	measured	-	-
magnetic flux density	5 mT	unspecified	measured	-	-

Measurement and calculation details

The magnetic field was measured with a Teslometer.

Methods Endpoint/measurement parameters/methodology

genotoxicity/mutation: DNA damage (content of 8-hydroxy-2-deoxyguanosine (high performance liquid chromatography))

Investigated system:

DNA hydrolysate, nucleosides

Time of investigation:

after exposure

Main outcome of study (acc. to author)

No statistical significant differences in the 8-hydroxy-2-deoxyguanosine contents were observed.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

State Committee for Scientific Research, Poland

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